Npy antibody

Antigen: Npy

Synonyms: 0710005A05Rik

Clonality: Polyclonal

Host: Goat

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN350525

Additional Information

Alternative name: NPY (Neuropeptide Y, CPON)

UniProt: P57774

Immunogen: A synthetic peptide from the internal part of mouse NPY (Neuropeptide Y) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in many other species including human, rat, zebrafish and xenopus.

Format: Lyophilized

Description: Function: NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone. Subcellular location: Secreted Tissue specificity: One of the most abundant peptides in the nervous system. Also found in some chromaffin cells of the adrenal medulla. Also known as: Neuropeptide Y, Neuropeptide tyrosine, C-flanking peptide of NPY, CPON.

Specificity: Appears to be specific for NPY.

Application Details

Application Notes: IHC (cryo and paraffin sections), WB. A dilution of 1: 300 to 1: 3000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Purification: Protein A affinity column

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Minth, Andrews, Dixon: "Characterization, sequence, and expression of the cloned human neuropeptide Y gene." in: The Journal of biological chemistry, Vol. 261, Issue 26, pp. 11974-9, 1986 (PubMed).

Minth, Bloom, Polak et al.: "Cloning, characterization, and DNA sequence of a human cDNA encoding neuropeptide tyrosine." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 81, Issue 14, pp. 4577-81, 1984 (PubMed).

Taylor, Wnek, Schroeder et al.: "Multiple obesity QTLs identified in an intercross between the NZO (New Zealand obese) and the SM (small) mouse strains." in: Mammalian genome : official journal of the International Mammalian Genome Society, Vol. 12, Issue 2, pp. 95-103, 2001 (PubMed).

Hillier, Fulton, Fulton et al.: "The DNA sequence of human chromosome 7." in: Nature, Vol. 424, Issue 6945, pp. 157-64, 2003 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).