Vacuolar protein sorting-associated protein 35 (Vesicle protein sorting 35, VPS35, MEM3) (N-Term) antibody

Antigen: Vacuolar protein sorting-associated protein 35 (Vesicle protein sorting 35, VPS35, MEM3)

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350530

Additional Information

UniProt: Q96QK1

Immunogen: A synthetic peptide from the n-terminal human Vacuolar protein sorting-associated protein 35 (Vesicle protein sorting 35, VPS35, MEM3) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat, mouse.

Format: Lyophilized

Isotype: IgG

Description: FUNCTION: Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein. TISSUE SPECIFICITY: Ubiquitous. Highly expressed in heart, brain, placenta, skeletal muscle, spleen, thymus, testis, ovary, small intestine, kidney and colon. Also known as: Vesicle protein sorting 35, Maternal-embryonic 3, VPS35, MEM3.

Specificity: Appears to be specific for VPS35.

Application Details

Application Notes: IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not tested in other applications.

Purity: IgG

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Edgar, Polak: "Human homologues of yeast vacuolar protein sorting 29 and 35." in: Biochemical and biophysical research communications, Vol. 277, Issue 3, pp. 622-30, 2000 (PubMed).

Haft, de la Luz Sierra, Bafford et al.: "Human orthologs of yeast vacuolar protein sorting proteins Vps26, 29, and 35: assembly into multimeric complexes." in: Molecular biology of the cell, Vol. 11, Issue 12, pp. 4105-16, 2000 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Vergues, Luton, Gruber et al.: "The mammalian retromer regulates transcytosis of the polymeric immunoglobulin receptor." in: Nature cell biology, Vol. 6, Issue 8, pp. 763-9, 2004 (PubMed).

Hierro, Rojas, Rojas et al.: "Functional architecture of the retromer cargo-recognition complex." in: Nature, Vol. 449, Issue 7165, pp. 1063-7, 2007 (PubMed).