Oligodendrocyte Lineage Transcription Factor 2 (OLIG2) (N-Term) antibody

Details for Product No. ABIN350657
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Antigen
Synonyms BHLHB1, OLIGO2, RACK17, PRKCBP2, bHLHe19, AI604895, Bhlhb1, Olg-2, Oligo2, RK17, wu:fc64g09, olig3.2, MGC89336, OLIG2
Epitope
N-Term
(14), (8), (6), (5), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(81), (40), (36), (14), (13), (12), (12), (3)
Host
Rabbit
(74), (15), (1), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(78), (38), (33), (15), (15), (12), (10), (7), (4), (4), (2), (1)
Pubmed 4 references available
Catalog no. ABIN350657
Quantity 500 µg
Price
454.67 $   Plus shipping costs $45.00
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Immunogen A synthetic peptide from the n-terminal region of human OLIG2 (Oligo2, bHLHB1, RACK17) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat and mouse.
Isotype IgG
Specificity Specific for contactin-1.
Purification Purified mouse LN T cells
IgG
Alternative Name OLIG2 (Oligo2, bHLHB1, RACK17)
Background Function: Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.
Subcellular location: Nucleus. Cytoplasm
Tissue specificity: Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable. Involvement in disease: A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14,21)(q11.2,q22) with TCRA. Also known as: Basic helix loop helix protein class B 1, BHLHB1, olig2, OLIGO2, Oligodendrocyte specific bHLH transcription factor 2, Oligodendrocyte transcription factor 2, PRKCBP2, Protein kinase C binding protein 2, Protein kinase C binding protein RACK17, RACK17.
UniProt Q63198
Research Area Neurology
Application Notes A concentration of 10-50 µg/mL is recommended.
The optimal concentration should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage -20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20 °C for long term storage and refrigerated at 2-8 °C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability.
Expiry Date 12 months
Supplier Images
anti-Oligodendrocyte Lineage Transcription Factor 2 (OLIG2) (N-Term) antibody WB on mouse brain lysate (denatured, reduced) using Rabbit antibody to n-terminal region of OLIG2 (Oligo2, bHLHB1, RACK17): IgG (ABIN350657) at a concentration of 30 µg/ml. Blocked with 1% LFDM for 15 minutes at room temperature with shake, primary antibody incubated for 15 minutes at room temperature, washed 3 times with PBST, 5 minutes each. Secondary antibody was also incubated for 15 minutes at room temperature.
General Wang, Jani-Sait, Escalon et al.: "The t(14;21)(q11.2;q22) chromosomal translocation associated with T-cell acute lymphoblastic leukemia activates the BHLHB1 gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 7, pp. 3497-502, 2000 (PubMed).

Marie, Sanson, Mokhtari et al.: "OLIG2 as a specific marker of oligodendroglial tumour cells." in: Lancet, Vol. 358, Issue 9278, pp. 298-300, 2001 (PubMed).

Lu, Park, Noll et al.: "Oligodendrocyte lineage genes (OLIG) as molecular markers for human glial brain tumors." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 19, pp. 10851-6, 2001 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Hosts (74), (15), (1), (1)
Reactivities (81), (40), (36), (14), (13), (12), (12), (3)
Applications (78), (38), (33), (15), (15), (12), (10), (7), (4), (4), (2), (1)
Conjugates (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (14), (8), (6), (5), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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