Opsin 1 (AA 2-47) antibody

Details for Product No. ABIN350673
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Antigen
Epitope
AA 2-47
Reactivity
Mouse (Murine), Rat (Rattus)
Host
Rabbit
Clonality
Polyclonal
Application
Immunohistochemistry (IHC), Western Blotting (WB)
Pubmed 4 references available
Quantity 100 μL
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Catalog No. ABIN350673
454.67 $
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Immunogen A synthetic peptide from aa region 2-47 of mouse Opsin 1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat.
Specificity Specific for Opn1mw.
Purification Whole serum
Background Function: Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal. May increase spectral sensitivity in dim light.
Subcellular location: Membrane, Multi-pass membrane protein.
Tissue specificity: Expresssed in cone photoreceptor cells. Also known as: Green-sensitive opsin, green cone photoreceptor pigment, medium wavelength-sensitive cone opsin, M opsin, Opsin 1, Gcp.
Research Area Neurology
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
General Sun, Macke, Nathans: "Mechanisms of spectral tuning in the mouse green cone pigment." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 16, pp. 8860-5, 1997 (PubMed).

Yokoyama, Radlwimmer: "The "five-sites" rule and the evolution of red and green color vision in mammals." in: Molecular biology and evolution, Vol. 15, Issue 5, pp. 560-7, 1998 (PubMed).

Radlwimmer, Yokoyama: "Genetic analyses of the green visual pigments of rabbit (Oryctolagus cuniculus) and rat (Rattus norvegicus)." in: Gene, Vol. 218, Issue 1-2, pp. 103-9, 1998 (PubMed).

Applebury, Antoch, Baxter et al.: "The murine cone photoreceptor: a single cone type expresses both S and M opsins with retinal spatial patterning." in: Neuron, Vol. 27, Issue 3, pp. 513-23, 2000 (PubMed).

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