Opsin 3 (OPN3) antibody

Antigen: Opsin 3 (OPN3)

Synonyms: ERO, Ecpn, panopsin, MGC124138, ECPN, OPN3

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350680

Additional Information

Alternative name: Opsin-3 (OPN3, ECPN, encephalopsin, panopsin)

UniProt: Q9H1Y3

Immunogen: A synthetic peptide as a part of human opsin-3 (OPN3, ECPN, encephalopsin, panopsin) conjugated to immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: Opsins are members of the guanine nucleotide-binding protein (G protein)-coupled receptor superfamily. In addition to the visual opsins, mammals possess several photoreceptive non-visual opsins that are expressed in extraocular tissues. This gene, opsin 3, is strongly expressed in brain and testis and weakly expressed in liver, placenta, heart, lung, skeletal muscle, kidney, and pancreas. The gene may also be expressed in the retina. The protein has the canonical features of a photoreceptive opsin protein. FUNCTION: May play a role in encephalic photoreception. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Strongly expressed in brain. Highly expressed in the preoptic area and paraventricular nucleus of the hypothalamus. Shows highly patterned expression in other regions of the brain, being enriched in selected regions of the cerebral cortex, cerebellar Purkinje cells, a subset of striatal neurons, selected thalamic nuclei, and a subset of interneurons in the ventral horn of the spinal cord. Also known as: Opsin-3, encephalopsin, panopsin, OPN3, ECPN, opsin 3.

Specificity: Appears to be specific for opsin-3.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Blackshaw, Snyder: "Encephalopsin: a novel mammalian extraretinal opsin discretely localized in the brain." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 10, pp. 3681-90, 1999 (PubMed).

Halford, Freedman, Bellingham et al.: "Characterization of a novel human opsin gene with wide tissue expression and identification of embedded and flanking genes on chromosome 1q43." in: Genomics, Vol. 72, Issue 2, pp. 203-8, 2001 (PubMed).

Kasper, Taudien, Staub et al.: "Different structural organization of the encephalopsin gene in man and mouse." in: Gene, Vol. 295, Issue 1, pp. 27-32, 2002 (PubMed).

Alam, Gorman, Jaeger et al.: "Germline deletions of EXO1 do not cause colorectal tumors and lesions which are null for EXO1 do not have microsatellite instability." in: Cancer genetics and cytogenetics, Vol. 147, Issue 2, pp. 121-7, 2003 (PubMed).

Gregory, Barlow, McLay et al.: "The DNA sequence and biological annotation of human chromosome 1." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006 (PubMed).