Opsin 4 (OPN4) antibody

Antigen: Opsin 4 (OPN4)

Synonyms: Gm533, 1110007J02Rik, MOP, MGC142118, opn4, OPN4, OPN4-2, opn4-A, Xmop, opn4d

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350682

Additional Information

Alternative name: opsin-4 (OPN4, melanopsin, MOP, Mopn)

UniProt: Q9QXZ9

Immunogen: A synthetic peptide as a part of mouse opsin-4 (OPN4, melanopsin, MOP, Mopn) conjugated to immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: Opsins are members of the guanine nucleotide-binding protein (G protein)-coupled receptor superfamily. This gene encodes a photoreceptive opsin protein that is expressed within the ganglion and amacrine cell layers of the retina. In mouse, retinal ganglion cell axons expressing this gene projected to the suprachiasmatic nucleus and other brain nuclei involved in circadian photoentrainment. In mouse, this protein is coupled to a transient receptor potential (TRP) ion channel through a G protein signaling pathway and produces a physiologic light response via membrane depolarization and increased intracellular calcium. The protein functions as a sensory photopigment and may also have photoisomerase activity. Experiments with knockout mice indicate that this gene attenuates, but does not abolish, photoentrainment. Alternative splicing results in multiple transcript variants encoding different isoforms. FUNCTION: Photoreceptor required for regulation of circadian rhythm. Contributes to pupillar reflex and other non-image forming responses to light. May be able to isomerize covalently bound all-trans retinal back to 11-cis retinal. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Note: Found in soma, dendrites and proximal part of axons of certain retinal ganglion cells . TISSUE SPECIFICITY: Eye. Expression is restricted within the ganglion and amacrine cell layers of the retina. Also known as: Similar to melanopsin, opsin-4, OPN4, melanopsin, MOP, Mopn, LOC721663.

Specificity: Appears to be specific for opsin-4.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Provencio, Rodriguez, Jiang et al.: "A novel human opsin in the inner retina." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 20, Issue 2, pp. 600-5, 2000 (PubMed).

Hattar, Lucas, Mrosovsky et al.: "Melanopsin and rod-cone photoreceptive systems account for all major accessory visual functions in mice." in: Nature, Vol. 424, Issue 6944, pp. 76-81, 2003 (PubMed).