ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1) (C-Term) antibody

Details for Product No. ABIN350685
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Antigen
Synonyms im:7146264, orai1, tmem142a, zgc:109721, orai2, ORAI1, LOC100218031, CRACM1, ORAT1, TMEM142A, D730049H07Rik, Tmem142a, orai-1, RGD1311873, tmem142b
Epitope
C-Term
(29), (13), (12), (8), (7), (3), (2), (1)
Reactivity
Human, Rat (Rattus), Mouse (Murine)
(80), (41), (23), (2), (2), (2), (2)
Host
Rabbit
(55), (19), (8)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(78), (54), (37), (13), (1)
Pubmed 5 references available
Quantity 100 µL
Options
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Catalog No. ABIN350685
454.67 $
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Immunogen A synthetic peptide from the c-terminal region of rat Orai1 (TMEM142a) conjugated to an immunogenic carrier protein was used as the antigen. The antigen is homologous in mouse and human.
Specificity Specific for Orai1.
Purification Whole serum
Alternative Name Orai1
Background Function: Ca2+ release-activated Ca2+ (CRAC) channel subunit which mediates Ca2+ influx following depletion of intracellular Ca2+ stores and channel activation by the Ca2+ sensor, STIM1. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. Defects in ORAI1 are a cause of severe combined immunodeficiency with CRAC channel dysfunction (CRAC-SCID). SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Subcellular location: Cell membrane, Multi-pass membrane protein. Also known as: Orai-1, TMEM142a, CRACM1, Calcium release-activated calcium channel protein 1,transmembrane protein 142A.
Research Area Signaling, Adaptive Immunity
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user. Not tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
General Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs. ..." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome. ..." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).

Feske, Gwack, Prakriya et al.: "A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function." in: Nature, Vol. 441, Issue 7090, pp. 179-85, 2006 (PubMed).

Soboloff, Spassova, Tang et al.: "Orai1 and STIM reconstitute store-operated calcium channel function." in: The Journal of biological chemistry, Vol. 281, Issue 30, pp. 20661-5, 2006 (PubMed).

Mercer, Dehaven, Smyth et al.: "Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1." in: The Journal of biological chemistry, Vol. 281, Issue 34, pp. 24979-90, 2006 (PubMed).

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