ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1) (C-Term) antibody

Antigen: ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1)

Synonyms: ORAT1, CRACM1, FLJ14466, TMEM142A, orai-1, Tmem142a, D730049H07Rik, RGD1311873, tmem142a, im:7146264, zgc:109721, orai2, MGC68830, ORAI1, MGC159526, LOC100218031

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350685

Additional Information

Alternative name: Orai1 (TMEM142a)

UniProt: Q5M848

Immunogen: A synthetic peptide from the c-terminal region of rat Orai1 (TMEM142a) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in mouse and human.

Format: Lyophilized

Description: FUNCTION: Ca2+ release-activated Ca2+ (CRAC) channel subunit which mediates Ca2+ influx following depletion of intracellular Ca2+ stores and channel activation by the Ca2+ sensor, STIM1. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. Defects in ORAI1 are a cause of severe combined immunodeficiency with CRAC channel dysfunction (CRAC-SCID). SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Also known as: Orai-1, TMEM142a, CRACM1, Calcium release-activated calcium channel protein 1,transmembrane protein 142A.

Specificity: Appears to be specific for Orai1.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).

Feske, Gwack, Prakriya et al.: "A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function." in: Nature, Vol. 441, Issue 7090, pp. 179-85, 2006 (PubMed).

Soboloff, Spassova, Tang et al.: "Orai1 and STIM reconstitute store-operated calcium channel function." in: The Journal of biological chemistry, Vol. 281, Issue 30, pp. 20661-5, 2006 (PubMed).

Mercer, Dehaven, Smyth et al.: "Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1." in: The Journal of biological chemistry, Vol. 281, Issue 34, pp. 24979-90, 2006 (PubMed).