Hypocretin (Orexin) Receptor 1 (HCRTR1) (internal) antibody

Antigen: Hypocretin (Orexin) Receptor 1 (HCRTR1)

Synonyms: OX1R, LOC443167, HCRTR1, Hctr1, Ox1r, MGC141357

Epitope: internal

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350687

Additional Information

Alternative name: Orexin Receptor 1 (OX1R) (internal region)

Swiss-Prot: P58307

Immunogen: A synthetic peptide from the internal region of mouse Orexin Receptor 1 (OX1R) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat.

Format: Lyophilized

Description: FUNCTION: Moderately selective excitatory receptor for orexin-A and, with a lower affinity, for orexin-B neuropeptide. Seems to be exclusively coupled to the G(q) subclass of heteromeric G proteins, which activates the phospholipase C mediated signaling cascade. Subcellular location: Cell membrane; Multi-pass membrane protein. Also known as: Orexin receptor type 1, OX1R, Hypocretin receptor type 1.

Specificity: Appears to be specific for Orexin Receptor 1.

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Sakurai, Amemiya, Ishii et al.: "Orexins and orexin receptors: a family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior." in: Cell, Vol. 92, Issue 4, pp. 573-85, 1998 (PubMed).

Willie, Chemelli, Sinton et al.: "To eat or to sleep? Orexin in the regulation of feeding and wakefulness." in: Annual review of neuroscience, Vol. 24, pp. 429-58, 2001 (PubMed).

Hungs, Mignot: "Hypocretin/orexin, sleep and narcolepsy." in: BioEssays : news and reviews in molecular, cellular and developmental biology, Vol. 23, Issue 5, pp. 397-408, 2001 (PubMed).