Hypocretin (Orexin) Receptor 1 (HCRTR1) (AA 2-46) antibody

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Antigen
  • HCRTR1
  • OX1R
  • Ox1r
  • Hctr1
  • hypocretin (orexin) receptor 1
  • orexin receptor 1
  • orexin receptor type 1-like
  • HCRTR1
  • LOC100352294
  • Hcrtr1
  • LOC443167
  • LOC100855896
Alternatives
anti-Human Hypocretin (Orexin) Receptor 1 antibody for ELISA
Epitope
AA 2-46
16
15
11
10
5
4
3
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
83
35
28
6
6
5
5
5
4
2
2
1
1
Host
Rabbit
76
8
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
2
2
2
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
55
39
24
6
4
2
2
Options
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Immunogen A synthetic peptide from aa region 2-46 of human Orexin Receptor 1 conjugated to blue carrier protein was used as the antigen.
Specificity Specific for Orexin Receptor 1.
Purification Whole serum
Alternative Name OX1R (HCRTR1 Antibody Abstract)
Background Function: Moderately selective excitatory receptor for orexin-A and, with a lower affinity, for orexin-B neuropeptide. Seems to be exclusively coupled to the G(q) subclass of heteromeric G proteins, which activates the phospholipase C mediated signaling cascade.
Subcellular location: Cell membrane, Multi-pass membrane protein. Also known as: Orexin receptor type 1, OX1R, Hypocretin receptor type 1.
Pathways Feeding Behaviour
Application Notes A dilution of 1: 300 to 1: 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Rikova, Guo, Zeng, Possemato, Yu, Haack, Nardone, Lee, Reeves, Li, Hu, Tan, Stokes, Sullivan, Mitchell, Wetzel, Macneill, Ren, Yuan, Bakalarski, Villen, Kornhauser, Smith, Li, Zhou, Gygi, Gu et al.: "Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer. ..." in: Cell, Vol. 131, Issue 6, pp. 1190-203, 2007 (PubMed).

Gregory, Barlow, McLay, Kaul, Swarbreck, Dunham, Scott, Howe, Woodfine, Spencer, Jones, Gillson, Searle, Zhou, Kokocinski, McDonald, Evans, Phillips, Atkinson, Cooper, Jones, Hall, Andrews, Lloyd et al.: "The DNA sequence and biological annotation of human chromosome 1. ..." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006 (PubMed).

Olafsdóttir, Rye, Scammell, Matheson, Stefánsson, Gulcher: "Polymorphisms in hypocretin/orexin pathway genes and narcolepsy." in: Neurology, Vol. 57, Issue 10, pp. 1896-9, 2001 (PubMed).

Peyron, Faraco, Rogers, Ripley, Overeem, Charnay, Nevsimalova, Aldrich, Reynolds, Albin, Li, Hungs, Pedrazzoli, Padigaru, Kucherlapati, Fan, Maki, Lammers, Bouras, Kucherlapati, Nishino, Mignot: "A mutation in a case of early onset narcolepsy and a generalized absence of hypocretin peptides in human narcoleptic brains." in: Nature medicine, Vol. 6, Issue 9, pp. 991-7, 2000 (PubMed).

Sakurai, Amemiya, Ishii, Matsuzaki, Chemelli, Tanaka, Williams, Richardson, Kozlowski, Wilson, Arch, Buckingham, Haynes, Carr, Annan, McNulty, Liu, Terrett, Elshourbagy, Bergsma, Yanagisawa: "Orexins and orexin receptors: a family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior." in: Cell, Vol. 92, Issue 4, pp. 573-85, 1998 (PubMed).