Hypocretin (Orexin) Receptor 1 (HCRTR1) (N-Term) antibody

Antigen: Hypocretin (Orexin) Receptor 1 (HCRTR1)

Synonyms: OX1R, LOC443167, HCRTR1, Hctr1, Ox1r, MGC141357

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350688

Additional Information

Alternative name: Orexin Receptor 1 (OX1R) (n-terminal region)

Swiss-Prot: O43613

Immunogen: A synthetic peptide from the n-terminal region of human Orexin Receptor 1 (OX1R) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Moderately selective excitatory receptor for orexin-A and, with a lower affinity, for orexin-B neuropeptide. Seems to be exclusively coupled to the G(q) subclass of heteromeric G proteins, which activates the phospholipase C mediated signaling cascade. Subcellular location: Cell membrane; Multi-pass membrane protein. Also known as: Orexin receptor type 1, OX1R, Hypocretin receptor type 1.

Specificity: Appears to be specific for Orexin Receptor 1.

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Sakurai, Amemiya, Ishii et al.: "Orexins and orexin receptors: a family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior." in: Cell, Vol. 92, Issue 4, pp. 573-85, 1998 (PubMed).

Peyron, Faraco, Rogers et al.: "A mutation in a case of early onset narcolepsy and a generalized absence of hypocretin peptides in human narcoleptic brains." in: Nature medicine, Vol. 6, Issue 9, pp. 991-7, 2000 (PubMed).

Olafsduottir, Rye, Scammell et al.: "Polymorphisms in hypocretin/orexin pathway genes and narcolepsy." in: Neurology, Vol. 57, Issue 10, pp. 1896-9, 2001 (PubMed).

Gregory, Barlow, McLay et al.: "The DNA sequence and biological annotation of human chromosome 1." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006 (PubMed).

Rikova, Guo, Zeng et al.: "Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." in: Cell, Vol. 131, Issue 6, pp. 1190-203, 2007 (PubMed).