Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (C-Term), (AA 100-170) antibody

Details for Product No. ABIN350689
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Antigen
Synonyms
CDKN1B, CIB, CIBP, KIP1, PRKDCIP, SIP2-28, p27Kip1, AA408329, AI843786, Kip1, p27, CDKN4, Cdki1b, P27KIP1, MEN1B, MEN4, cdkn1bl, cdkn1b, sb:cb611, wu:fb63g10, wu:fb64g10, wu:fe18e03, FAM14D, ISG12, IS ... show more
CDKN1B, CIB, CIBP, KIP1, PRKDCIP, SIP2-28, p27Kip1, AA408329, AI843786, Kip1, p27, CDKN4, Cdki1b, P27KIP1, MEN1B, MEN4, cdkn1bl, cdkn1b, sb:cb611, wu:fb63g10, wu:fb64g10, wu:fe18e03, FAM14D, ISG12, ISG12A, P27, 1500011J20Rik, 2610202L11Rik, Bridge-1 show less
Epitope
C-Term, AA 100-170
(70), (57), (36), (36), (29), (21), (13), (11), (11), (10), (9), (9), (9), (8), (7), (7), (6), (6), (6), (6), (6), (5), (4), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus), Mouse (Murine)
(428), (183), (142), (80), (61), (53), (36), (14), (8), (3), (3), (1), (1), (1), (1)
Host
Rabbit
(399), (66), (14), (2), (1), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(21), (19), (17), (11), (11), (11), (6), (6), (6), (6), (6), (6), (6), (6), (2), (2)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(323), (180), (98), (97), (60), (60), (52), (30), (25), (16), (13), (8), (6), (6), (1)
Pubmed 5 references available
Quantity 100 μL
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Catalog No. ABIN350689
454.67 $
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Immunogen A synthetic peptide from c-terminal region (~aa 100-170) of human p27 Kip1 (KIP1, p27Kip1) conjugated to an immunogenic carrier protein was used as the antigen.
Specificity Specific for p27Kip1.
Purification Whole serum
Alternative Name p27 Kip1
Background Function: Important regulator of cell cycle progrssion. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Positive regulator of cyclin D-dependent kinases such as CDK4. Regulated by phosphorylation and degradation events. Decreased levels of p27Kip1, mainly due to proteosomal degradation, are found in various epithelial tumors originating from lung, breast, colon, ovary, esophagus, thyroid and prostate.
Subcellular location: Nucleus. Cytoplasm. Note: Nuclear and cytoplasmic in quiescent cells.
Tissue specificity: Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney. Also known as: Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, CDKN1B, KIP1, p27Kip1, p27 Kip 1.
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user. Not tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
General Pietenpol, Bohlander, Sato et al.: "Assignment of the human p27Kip1 gene to 12p13 and its analysis in leukemias." in: Cancer research, Vol. 55, Issue 6, pp. 1206-10, 1995 (PubMed).

Polyak, Lee, Erdjument-Bromage et al.: "Cloning of p27Kip1, a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals." in: Cell, Vol. 78, Issue 1, pp. 59-66, 1994 (PubMed).

Ishida, Kitagawa, Hatakeyama et al.: "Phosphorylation at serine 10, a major phosphorylation site of p27(Kip1), increases its protein stability." in: The Journal of biological chemistry, Vol. 275, Issue 33, pp. 25146-54, 2000 (PubMed).

Viglietto, Motti, Bruni et al.: "Cytoplasmic relocalization and inhibition of the cyclin-dependent kinase inhibitor p27(Kip1) by PKB/Akt-mediated phosphorylation in breast cancer." in: Nature medicine, Vol. 8, Issue 10, pp. 1136-44, 2002 (PubMed).

Grimmler, Wang, Mund et al.: "Cdk-inhibitory activity and stability of p27Kip1 are directly regulated by oncogenic tyrosine kinases." in: Cell, Vol. 128, Issue 2, pp. 269-80, 2007 (PubMed).

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