Purinergic Receptor P2X, Lig-Nd-Gated Ion Channel, 1 (P2RX1) (cytoplasmic domain) antibody

Antigen: Purinergic Receptor P2X, Lig-Nd-Gated Ion Channel, 1 (P2RX1)

Synonyms: P2X1

Epitope: cytoplasmic domain

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350691

Additional Information

Alternative name: P2X1 (P2RX1, P2X purinoceptor 1, ATP receptor) (cytoplasmic domain)

Swiss-Prot: P51575

Immunogen: A synthetic peptide from the cytoplasmic domain of human P2X1 (P2RX1, P2X purinoceptor 1, ATP receptor) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen shares 92% identity with rat and mouse sequence.

Format: Lyophilized

Description: FUNCTION: Ligand gated ion channel with relatively high calcium permeability. Binding to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle. Seems to be linked to apoptosis, by increasing the intracellular concentration of calcium in the presence of ATP, leading to programmed cell death. Subcellular location: Membrane; Multi-pass membrane protein. Involvement in disease: Defects in P2RX1 are a cause of bleeding disorder Also known as: P2RX1, P2X1, P2X purinoceptor 1, ATP receptor.

Specificity: Appears to be specific for P2RX1.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Longhurst, Schwegel, Folander et al.: "The human P2x1 receptor: molecular cloning, tissue distribution, and localization to chromosome 17." in: Biochimica et biophysica acta, Vol. 1308, Issue 3, pp. 185-8, 1996 (PubMed).

Valera, Talabot, Evans et al.: "Characterization and chromosomal localization of a human P2X receptor from the urinary bladder." in: Receptors & channels, Vol. 3, Issue 4, pp. 283-9, 1996 (PubMed).

Sun, Li, Okahara et al.: "P2X1 purinoceptor in human platelets. Molecular cloning and functional characterization after heterologous expression." in: The Journal of biological chemistry, Vol. 273, Issue 19, pp. 11544-7, 1998 (PubMed).

Oury, Toth-Zsamboki, Van Geet et al.: "A natural dominant negative P2X1 receptor due to deletion of a single amino acid residue." in: The Journal of biological chemistry, Vol. 275, Issue 30, pp. 22611-4, 2000 (PubMed).