P2X7 (P2RX7, P2X purinoceptor 7, Purinergic receptor) (Extracellular Domain) antibody

Antigen: P2X7 (P2RX7, P2X purinoceptor 7, Purinergic receptor)

Binding Site: Extracellular Domain

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350705

Additional Information

UniProt: Q9Z1M0

Immunogen: A synthetic peptide from the extracellular domain of mouse P2X7 (P2RX7, P2X purinoceptor 7, Purinergic receptor, P2Z receptor, ATP receptor) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in human and shares 95% identity with rat's.

Format: Lyophilized

Description: FUNCTION: Receptor for ATP that acts as a ligand gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. Subcellular location: Membrane; Multi-pass membrane protein. Also known as: P2RX7, P2X purinoceptor 7, Purinergic receptor, P2Z receptor, ATP receptor.

Specificity: Appears to be specific for P2RX7.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Rassendren, Buell, Virginio et al.: "The permeabilizing ATP receptor, P2X7. Cloning and expression of a human cDNA." in: The Journal of biological chemistry, Vol. 272, Issue 9, pp. 5482-6, 1997 (PubMed).

Buell, Talabot, Gos et al.: "Gene structure and chromosomal localization of the human P2X7 receptor." in: Receptors & channels, Vol. 5, Issue 6, pp. 347-54, 1999 (PubMed).

Chessell, Simon, Hibell et al.: "Cloning and functional characterisation of the mouse P2X7 receptor." in: FEBS letters, Vol. 439, Issue 1-2, pp. 26-30, 1998 (PubMed).

Gu, Zhang, Worthington et al.: "A Glu-496 to Ala polymorphism leads to loss of function of the human P2X7 receptor." in: The Journal of biological chemistry, Vol. 276, Issue 14, pp. 11135-42, 2001 (PubMed).

Sjöblom, Jones, Wood et al.: "The consensus coding sequences of human breast and colorectal cancers." in: Science (New York, N.Y.), Vol. 314, Issue 5797, pp. 268-74, 2006 (PubMed).