p75NTR (low affinity NGF receptor, p75, LNGFR, p75NTR) (Cytoplasmic Domain) antibody

Antigen: p75NTR (low affinity NGF receptor, p75, LNGFR, p75NTR)

Binding Site: Cytoplasmic Domain

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350710

Additional Information

UniProt: P08138

Immunogen: A synthetic peptide from the cytoplasmic domain of human p75NTR (low affinity NGF receptor, p75, LNGFR, p75NTR) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in many other species including mouse and rat.

Format: Lyophilized

Isotype: IgG

Description: FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/<||a href="http://www.osenses.com/bex-family-c-21_106.html" >BEX3<||/a>. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with <||a href="http://www.osenses.com/advanced_search_result.php?antigen=sqstm1&x=0&y=0" >SQSTM1<||/a> which bridges NGFR to NTRK1. Interacts with <||a href="http://www.osenses.com/bex-family-c-21_106.html" >BEX1<||/a>. Interacts with ARMS and NTRK1. Can form a ternary complex with NTRK1 and ARMS and this complex is affected by the expression levels of ARMS. An increase in ARMS expression leads to a decreased association of NGFR and NTRK1. Interacts with LINGO1. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. Also known as: Low-affinity nerve growth factor receptor, NGF receptor, Gp80-LNGFR, p75 ICD, Low affinity neurotrophin receptor p75NTR, p75, LNGFR, p75NTR, RNNGFRR.

Specificity: Appears to be specific for p75NTR.

Application Details

Application Notes: IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Purity: IgG

Purification: Purified

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Johnson, Lanahan, Buck et al.: "Expression and structure of the human NGF receptor." in: Cell, Vol. 47, Issue 4, pp. 545-54, 1986 (PubMed).

Chapman, Eckart, Kaufman et al.: "O-linked oligosaccharide on the 75-kDa neurotrophin receptor." in: Journal of neurochemistry, Vol. 66, Issue 4, pp. 1707-16, 1996 (PubMed).

Khursigara, Orlinick, Chao: "Association of the p75 neurotrophin receptor with TRAF6." in: The Journal of biological chemistry, Vol. 274, Issue 5, pp. 2597-600, 1999 (PubMed).

Ye, Mehlen, Rabizadeh et al.: "TRAF family proteins interact with the common neurotrophin receptor and modulate apoptosis induction." in: The Journal of biological chemistry, Vol. 274, Issue 42, pp. 30202-8, 1999 (PubMed).

Irie, Hachiya, Rabizadeh et al.: "Functional interaction of Fas-associated phosphatase-1 (FAP-1) with p75(NTR) and their effect on NF-kappaB activation." in: FEBS letters, Vol. 460, Issue 2, pp. 191-8, 1999 (PubMed).

Wooten, Seibenhener, Mamidipudi et al.: "The atypical protein kinase C-interacting protein p62 is a scaffold for NF-kappaB activation by nerve growth factor." in: The Journal of biological chemistry, Vol. 276, Issue 11, pp. 7709-12, 2001 (PubMed).

Mi, Lee, Shao et al.: "LINGO-1 is a component of the Nogo-66 receptor/p75 signaling complex." in: Nature neuroscience, Vol. 7, Issue 3, pp. 221-8, 2004 (PubMed).