Painless (PAIN) (4th Cytoplasmic Loop) antibody

Antigen: Painless (PAIN)

Synonyms: CG15860, CT31987, DmelCG15860, EP2251, GLEAN_07561, GB10136, NV14270

Binding Site: 4th Cytoplasmic Loop

Clonality: Polyclonal

Host: Rabbit

Reactivity: Fruit Fly (Drosophila melanogaster)

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350717

Additional Information

Alternative name: Pain (Transient receptor potential cation channel protein painless)

UniProt: Q9W0Y6

Immunogen: A synthetic peptide from 4th cytoplasmic loop of drosophila Pain (Transient receptor potential cation channel protein painless) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Receptor-activated non-selective cation channel involved in detection of pain sensation due to high temperature. Involved in heat nociception by being activated by noxious temperature of 38 degrees Celsius. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Present in multidendritic neurons, chordotonal neurons, a subset of cells in the central nervous system and a subset of sensory neurons in the antennal-maxillary complex. Not detected in gonads and dorsal vessels (at protein level). Expressed in peripheral neurons that extend multiple branched dendrites beneath the larval epidermis, similar to vertebrate pain receptors. DEVELOPMENTAL STAGE: Expressed from stage 13 of embryonic development in a small number of cells in the central nervous system and in a subset of neurons of the peripheral nervous system. Such cells may include sensory precursors of multidendritic (md) neurons. At embryonic stage 16, prior to when dendritic process are elaborated, it appears to be expressed in multidendritic neurons in a polarized manner. At stage 17 of development, when the md neurons first initiate dendritogenesis, it is localized to branched projections initiating from clusters of multidendritic neurons. Also known as: Transient receptor potential cation channel protein painless.

Specificity: Appears to be specific for Pain (Transient receptor potential cation channel protein painless).

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in othr applications.

Purity: whole serum

Purification: Purified

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Adams, Celniker, Holt et al.: "The genome sequence of Drosophila melanogaster." in: Science (New York, N.Y.), Vol. 287, Issue 5461, pp. 2185-95, 2000 (PubMed).

Tracey, Wilson, Laurent et al.: "painless, a Drosophila gene essential for nociception." in: Cell, Vol. 113, Issue 2, pp. 261-73, 2003 (PubMed).