Pan AKT (C-Term) antibody

Details for Product No. ABIN350718
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Antigen
Epitope
C-Term
(1)
Reactivity
Mouse (Murine), Rat (Rattus), Human
(1), (1), (1)
Host
Rabbit
(1)
Clonality
Polyclonal
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(1), (1)
Pubmed 5 references available
Quantity 100 µL
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Catalog No. ABIN350718
454.67 $
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Immunogen A synthetic peptide from the c-terminal of mouse AKT1 (RAC-alpha serine/threonine-protein kinase, PKB, RAC) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat, human and chicken.
Specificity Specific for AKT1, AKT2, AKT3.
Purification Whole serum
Background Function: General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation: Three specific sites, one in the kinase domain (Thr-308) and the two other ones in the C-terminal regulatory region (Ser-473 and Tyr-474), need to be phosphorylated for its full activation.
Subcellular location: Cytoplasm. Nucleus. Cell membrane. Note: Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A.
Tissue specificity: Widely expressed. Low levels found in liver with slightly higher levels present in thymus and testis. Also known as: RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, PKB, RAC, AKT1.
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Supplier Images
anti-Pan AKT (C-Term) antibody WB on rat brain lysate using Rabbit antibody to c-terminal of AKT (RAC-alpha serine/threonine-protein kinase, PKB, RAC): whole serum (ABIN350718) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.
General Cong, Chen, Li et al.: "Physiological role of Akt in insulin-stimulated translocation of GLUT4 in transfected rat adipose cells." in: Molecular endocrinology (Baltimore, Md.), Vol. 11, Issue 13, pp. 1881-90, 1998 (PubMed).

Pekarsky, Koval, Hallas et al.: "Tcl1 enhances Akt kinase activity and mediates its nuclear translocation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 7, pp. 3028-33, 2000 (PubMed).

Maira, Galetic, Brazil et al.: "Carboxyl-terminal modulator protein (CTMP), a negative regulator of PKB/Akt and v-Akt at the plasma membrane." in: Science (New York, N.Y.), Vol. 294, Issue 5541, pp. 374-80, 2001 (PubMed).

Anai, Shojima, Katagiri et al.: "A novel protein kinase B (PKB)/AKT-binding protein enhances PKB kinase activity and regulates DNA synthesis." in: The Journal of biological chemistry, Vol. 280, Issue 18, pp. 18525-35, 2005 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

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