Rho-related GTP-binding protein antibody

Catalog No. ABIN350723

Product Details anti-Rho-related GTP-binding protein Antibody

Target: Rho-related GTP-binding protein (RhO (pan))

Reactivity: Human, Mouse, Rat

Host: Sheep

Clonality: Polyclonal

Conjugate: This Rho-related GTP-binding protein antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Specific for RhO family.

Immunogen: A synthetic peptide as a part of human/rat/mouse/chicken/dog/frog (and more species) Rho-related GTP-binding proteins (RhOA, RhOB, RhOC, RhOF, RhOQ) and RAC1, RAC2, RAC3 and CDC42 conjugated to an immunogenic carrier protein was used as the immunogen.

Isotype: IgG

Application Details

Application Notes: A concentration of 10-50 µg/ml is recommended.
The optimal concentration should be determined by the end user.
Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for Rho-related GTP-binding protein

Target: Rho-related GTP-binding protein (RhO (pan))

Abstract: RhO (pan) Products

Target Type: Chemical

Background: The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Also known as: Rho-related GTP-binding protein RhOA, RhOB, RhOC, RhOF, RhOQ, RAC1, RAC2, RAC3, CDC42.