LIMK2 antibody (LIM Domain Kinase 2) (pSer291)

Details for Product anti-LIMK2 Antibody No. ABIN350732, Supplier: Log in to see
Antigen
  • xlimk2
  • zgc:91990
  • wu:fa97c09
  • wu:fk73e11
  • LIMK2
  • Limk2b
  • Link2
  • LIMK
  • 9430059K01
  • A930024P04Rik
  • C85310
  • Limk2a
  • LIM domain kinase 2
  • similar to LIM domain kinase 2
  • LIM motif-containing protein kinase 2
  • limk2
  • LIMK2
  • LOC100223808
  • Limk2
Epitope
pSer291, pSer293
30
15
11
8
8
5
5
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine), Rat (Rattus), Human
137
84
78
2
1
1
1
1
1
1
1
1
Host
Sheep
125
6
3
2
1
Clonality
Polyclonal
Conjugate
This LIMK2 antibody is un-conjugated
2
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
123
57
45
39
27
13
3
3
1
1
1
1
Options
Supplier
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Immunogen A synthetic peptide from the phospho S291 & S293 region of human LIMK2 (LIM domain kinase 2, LIMK-2) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat, mouse.
Isotype IgG
Specificity Specific for LIMK2.
Alternative Name LIMK2 (LIMK2 Antibody Abstract)
Background Function: Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Subcellular location: Cytoplasm. Nucleus. Note: Isoform LIMK2a is distributed in the cytoplasm and the nucleus, and isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.
Tissue specificity: Highest expression in the placenta, moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed. Also known as: LIM domain kinase 2, LIMK-2.
Application Notes A concentration of 10-50 µg/ml is recommended.
The optimal dilution should be determined by the end user. This antibody may also recognise the unphosphorylated form.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Sumi, Matsumoto, Nakamura: "Specific activation of LIM kinase 2 via phosphorylation of threonine 505 by ROCK, a Rho-dependent protein kinase." in: The Journal of biological chemistry, Vol. 276, Issue 1, pp. 670-6, 2001 (PubMed).

Dunham, Shimizu, Roe, Chissoe, Hunt, Collins, Bruskiewich, Beare, Clamp, Smink, Ainscough, Almeida, Babbage, Bagguley, Bailey, Barlow, Bates, Beasley, Bird, Blakey, Bridgeman, Buck, Burgess, Burrill et al.: "The DNA sequence of human chromosome 22. ..." in: Nature, Vol. 402, Issue 6761, pp. 489-95, 1999 (PubMed).

Maekawa, Ishizaki, Boku, Watanabe, Fujita, Iwamatsu, Obinata, Ohashi, Mizuno, Narumiya: "Signaling from Rho to the actin cytoskeleton through protein kinases ROCK and LIM-kinase." in: Science (New York, N.Y.), Vol. 285, Issue 5429, pp. 895-8, 1999 (PubMed).

Osada, Hasada, Inazawa, Uchida, Ueda, Takahashi, Takahashi: "Subcellular localization and protein interaction of the human LIMK2 gene expressing alternative transcripts with tissue-specific regulation." in: Biochemical and biophysical research communications, Vol. 229, Issue 2, pp. 582-9, 1997 (PubMed).

Okano, Hiraoka, Otera, Nunoue, Ohashi, Iwashita, Hirai, Mizuno: "Identification and characterization of a novel family of serine/threonine kinases containing two N-terminal LIM motifs." in: The Journal of biological chemistry, Vol. 270, Issue 52, pp. 31321-30, 1996 (PubMed).

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