LIMK2 (LIM domain kinase 2, LIMK-2) (pSer291, pSer293) antibody

Antigen: LIMK2 (LIM domain kinase 2, LIMK-2)

Binding Site: pSer291, pSer293

Clonality: Polyclonal

Host: Sheep

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350732

Additional Information

UniProt: P53671

Immunogen: A synthetic peptide from the phospho S291 & S293 region of human LIMK2 (LIM domain kinase 2, LIMK-2) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat, mouse.

Format: Lyophilized

Isotype: IgG

Description: FUNCTION: Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Isoform LIMK2a is distributed in the cytoplasm and the nucleus, and isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus. TISSUE SPECIFICITY: Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed. Also known as: LIM domain kinase 2, LIMK-2.

Specificity: Appears to be specific for LIMK2.

Application Details

Application Notes: IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal dilution should be determined by the end user. This antibody may also recognise the unphosphorylated form. Not yet tested in other applications.

Purity: IgG

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Okano, Hiraoka, Otera et al.: "Identification and characterization of a novel family of serine/threonine kinases containing two N-terminal LIM motifs." in: The Journal of biological chemistry, Vol. 270, Issue 52, pp. 31321-30, 1996 (PubMed).

Osada, Hasada, Inazawa et al.: "Subcellular localization and protein interaction of the human LIMK2 gene expressing alternative transcripts with tissue-specific regulation." in: Biochemical and biophysical research communications, Vol. 229, Issue 2, pp. 582-9, 1997 (PubMed).

Maekawa, Ishizaki, Boku et al.: "Signaling from Rho to the actin cytoskeleton through protein kinases ROCK and LIM-kinase." in: Science (New York, N.Y.), Vol. 285, Issue 5429, pp. 895-8, 1999 (PubMed).

Dunham, Shimizu, Roe et al.: "The DNA sequence of human chromosome 22." in: Nature, Vol. 402, Issue 6761, pp. 489-95, 1999 (PubMed).

Sumi, Matsumoto, Nakamura: "Specific activation of LIM kinase 2 via phosphorylation of threonine 505 by ROCK, a Rho-dependent protein kinase." in: The Journal of biological chemistry, Vol. 276, Issue 1, pp. 670-6, 2001 (PubMed).