BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) (pS95) antibody

Antigen: BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3)

Synonyms: NIP3

Epitope: pS95

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350733

Additional Information

Alternative name: phospho S95 BNIP3 (BCL2/adenovirus E1B 19 kDa protein-interacting protein 3, NIP3)

Swiss-Prot: Q12983

Immunogen: A synthetic peptide from the human phospho S95 BNIP3 (BCL2/adenovirus E1B 19 kDa protein-interacting protein 3, NIP3) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Subcellular location: Mitochondrion membrane; Single-pass membrane protein Potential. Note: Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Also known as: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3, BNIP3, NIP3.

Specificity: Appears to be specific for BNIP3.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. This antibody may also recognise the unphosphorylated form of the BNIP3.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Boyd, Malstrom, Subramanian et al.: "Adenovirus E1B 19 kDa and Bcl-2 proteins interact with a common set of cellular proteins." in: Cell, Vol. 79, Issue 2, pp. 341-51, 1994 (PubMed).

Chen, Ray, Dubik et al.: "The E1B 19K/Bcl-2-binding protein Nip3 is a dimeric mitochondrial protein that activates apoptosis." in: The Journal of experimental medicine, Vol. 186, Issue 12, pp. 1975-83, 1998 (PubMed).

Ohi, Tokunaga, Tsunoda et al.: "A novel adenovirus E1B19K-binding protein B5 inhibits apoptosis induced by Nip3 by forming a heterodimer through the C-terminal hydrophobic region." in: Cell death and differentiation, Vol. 6, Issue 4, pp. 314-25, 1999 (PubMed).

Deloukas, Earthrowl, Grafham et al.: "The DNA sequence and comparative analysis of human chromosome 10." in: Nature, Vol. 429, Issue 6990, pp. 375-81, 2004 (PubMed).