ERK1 / ERK2 antibody

Details for Product No. ABIN350735
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Antigen
Reactivity
Human, Rat (Rattus), Mouse (Murine)
(209), (139), (131), (83), (78), (71), (47), (24), (9), (9), (9), (3), (2), (1), (1), (1), (1), (1)
Host
Rabbit
(180), (34), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(9), (9), (6), (6), (6), (6), (6), (6), (6), (6), (6)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(148), (59), (48), (44), (39), (32), (19), (18), (10), (4), (3)
Pubmed 5 references available
Catalog no. ABIN350735
Quantity 100 µL
Price
454.67 $   Plus shipping costs $45.00
Options
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Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen A synthetic peptide from the phospho T202 & Y204 of human Erk1, Erk2 conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in many species including rat, mouse, zebra fish, xenopus and chicken.
Specificity Specific for QKI, isoforms 3, 5, 6,7.
Purification whole serum
Alternative Name Erk1, Erk2
Background Function: Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1, required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1. Phosphorylates heat shock factor protein 4 (HSF4).
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Cofactor: Magnesium.
Enzyme regulation: Activated by tyrosine phosphorylation in response to insulin and NGF.
Subunit: Interacts with MORG1. Binds to HIV-1 Nef. This interaction inhibits its kinase activity. Interacts with HSF4 and NISCH. Also known as: Mitogen-activated protein kinase 3, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 1, MAPK 1, p44-ERK1, ERT2, p44-MAPK, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, Extracellular signal-regulated kinase 2, ERK-2, Mitogen-activated protein kinase 2, MAP kinase 2, MAPK 2, p42-MAPK, ERT1, MAPK1, PRKM1, PRKM2, MAPK3, PRKM3.
UniProt Q96PU8
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user. This antibody may also recognise the unphosphorylated form.
Not yet tested in other applications.
Restrictions For Research Use only
Format Liquid
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage -20 °C
Storage Comment Maintain the reconstituted antibodies frozen at -20 °C for long term storage and refrigerated at 2-8 °C for a shorter term.
Expiry Date 12 months
General Charest, Mordret, Harder et al.: "Molecular cloning, expression, and characterization of the human mitogen-activated protein kinase p44erk1." in: Molecular and cellular biology, Vol. 13, Issue 8, pp. 4679-90, 1993 (PubMed).

Zhang, Wolf-Yadlin, Ross et al.: "Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules." in: Molecular & cellular proteomics : MCP, Vol. 4, Issue 9, pp. 1240-50, 2005 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Wissing, Jänsch, Nimtz et al.: "Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometry." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 3, pp. 537-47, 2007 (PubMed).

Rikova, Guo, Zeng et al.: "Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." in: Cell, Vol. 131, Issue 6, pp. 1190-203, 2007 (PubMed).

Hosts (180), (34), (3)
Reactivities (209), (139), (131), (83), (78), (71), (47), (24), (9), (9), (9), (3), (2), (1), (1), (1), (1), (1)
Applications (148), (59), (48), (44), (39), (32), (19), (18), (10), (4), (3)
Conjugates (9), (9), (6), (6), (6), (6), (6), (6), (6), (6), (6)
Epitopes (67), (64), (29), (23), (15), (12), (6), (6), (5), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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