TrkC (Trk-C, NTRK3, NT-3 growth factor receptor, GP145-TrkC) (pTyr820) antibody

Antigen: TrkC (Trk-C, NTRK3, NT-3 growth factor receptor, GP145-TrkC)

Binding Site: pTyr820

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350738

Additional Information

UniProt: Q16288

Immunogen: A synthetic peptide to phospho Y820 of human TrkC (Trk-C, NTRK3, NT-3 growth factor receptor, GP145-TrkC) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat and mouse.

Format: Lyophilized

Description: FUNCTION: Receptor for neurotrophin-3 (NT-3). This is a tyrosine-protein kinase receptor. Known substrates for the trk receptors are SHC1, PI-3 kinase, and PLCG1. The different isoforms do not have identical signaling properties. CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. Binds SH2B2. Interacts with SQSTM1 and ARMS. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Widely expressed, mainly in the nervous tissue. The isoform B is expressed in a relatively large amount in the adult brain comparatively to fetal brain. Also known as: Neurotrophic tyrosine kinase receptor type 3, TrkC tyrosine kinase, GP145-TrkC, Trk-C, NT-3 growth factor receptor, NTRK3.

Specificity: Appears to be specific for TrkC.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 3000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. This antibody may also recognise the unphosphorylated form.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

WB on rat brain lysate using Rabbit antibody to phospho Y820 TrkC (Trk-C, NTRK3, NT-3 growth factor receptor, GP145-TrkC): whole serum (ABIN350738) at 1 : 1000 dilution incubated overnight at 4°C. A single band will be detected. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.

Publications

Merlio, Ernfors, Jaber et al.: "Molecular cloning of rat trkC and distribution of cells expressing messenger RNAs for members of the trk family in the rat central nervous system." in: Neuroscience, Vol. 51, Issue 3, pp. 513-32, 1993 (PubMed).

McGregor, Baylin, Griffin et al.: "Molecular cloning of the cDNA for human TrkC (NTRK3), chromosomal assignment, and evidence for a splice variant." in: Genomics, Vol. 22, Issue 2, pp. 267-72, 1995 (PubMed).

Shelton, Sutherland, Gripp et al.: "Human trks: molecular cloning, tissue distribution, and expression of extracellular domain immunoadhesins." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 15, Issue 1 Pt 2, pp. 477-91, 1995 (PubMed).

Valenzuela, Maisonpierre, Glass et al.: "Alternative forms of rat TrkC with different functional capabilities." in: Neuron, Vol. 10, Issue 5, pp. 963-74, 1993 (PubMed).

Tsoulfas, Soppet, Escandon et al.: "The rat trkC locus encodes multiple neurogenic receptors that exhibit differential response to neurotrophin-3 in PC12 cells." in: Neuron, Vol. 10, Issue 5, pp. 975-90, 1993 (PubMed).

Qian, Riccio, Zhang et al.: "Identification and characterization of novel substrates of Trk receptors in developing neurons." in: Neuron, Vol. 21, Issue 5, pp. 1017-29, 1999 (PubMed).

Ultsch, Wiesmann, Simmons et al.: "Crystal structures of the neurotrophin-binding domain of TrkA, TrkB and TrkC." in: Journal of molecular biology, Vol. 290, Issue 1, pp. 149-59, 1999 (PubMed).

Ballif, Carey, Sunyaev et al.: "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." in: Journal of proteome research, Vol. 7, Issue 1, pp. 311-8, 2008 (PubMed).