Piccolo (Presynaptic Cytomatrix Protein) (PCLO) (internal) antibody

Antigen: Piccolo (Presynaptic Cytomatrix Protein) (PCLO)

Synonyms: Acz, Pico, mKIAA0559, ACZ, DKFZp779G1236, aczonin, pclo, wu:fj53e09

Epitope: internal

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350742

Additional Information

Alternative name: Piccolo (Aczonin) (internal region)

Swiss-Prot: Q9Y6V0

Immunogen: A synthetic peptide from internal part of human internal part of Piccolo (Aczonin) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat and mouse.

Format: Lyophilized

Description: FUNCTION: May act as a scaffolding protein involved in the organization of synaptic active zones and in synaptic vesicle trafficking. SUBUNIT: Interacts with Rabac1/Pra1, RIMS2 and profilin. SUBCELLULAR LOCATION: Cell junction, synapse. Note: Concentrated at the presynaptic side of synaptic junctions. TISSUE SPECIFICITY: Highly expressed in brain. Moderately expresssed in pituitary gland and pancreatic islets. Low levels found in stomach. Also known as: Aczonin.

Specificity: Appears to be specific for Piccolo.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

IF on 1 day-old mouse cortical cell culture using Rabbit antibody to internal part of Piccolo (Aczonin): whole serum (ABIN350742) at 1: 2000 dilution. DAPI counter stained.

IF on 1 day-old mouse cortical cell culture using Rabbit antibody to internal part of Piccolo (Aczonin): whole serum (ABIN350742) at 1: 2000 dilution. DAPI counter stained appearing in blue.

Publications

Publications: Wang, Kibschull, Laue et al.: "Aczonin, a 550-kD putative scaffolding protein of presynaptic active zones, shares homology regions with Rim and Bassoon and binds profilin." in: The Journal of cell biology, Vol. 147, Issue 1, pp. 151-62, 1999 (PubMed).

Fujimoto, Shibasaki, Yokoi et al.: "Piccolo, a Ca2+ sensor in pancreatic beta-cells. Involvement of cAMP-GEFII.Rim2. Piccolo complex in cAMP-dependent exocytosis." in: The Journal of biological chemistry, Vol. 277, Issue 52, pp. 50497-502, 2002 (PubMed).

Collins, Yu, Coba et al.: "Proteomic analysis of in vivo phosphorylated synaptic proteins." in: The Journal of biological chemistry, Vol. 280, Issue 7, pp. 5972-82, 2005 (PubMed).

Trinidad, Specht, Thalhammer et al.: "Comprehensive identification of phosphorylation sites in postsynaptic density preparations." in: Molecular & cellular proteomics : MCP, Vol. 5, Issue 5, pp. 914-22, 2006 (PubMed).

Munton, Tweedie-Cullen, Livingstone-Zatchej et al.: "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 2, pp. 283-93, 2007 (PubMed).