Polycystic Kidney Disease 2 (Autosomal Dominant) (PKD2) (4th Cytoplasmic Loop) antibody

Antigen: Polycystic Kidney Disease 2 (Autosomal Dominant) (PKD2)

Binding Site: 4th Cytoplasmic Loop

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350745

Additional Information

Alternative name: polycystic kidney disease 2 protein (PKD2, Polycystin-2)

UniProt: Q13563

Immunogen: A synthetic peptide from the 4th cytoplasmic loop of human Polycystic kidney disease 2 protein (PKD2, Polycystin-2, Polycystwin) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Functions as a calcium permeable cation channel. PKD1 and PKD2 may function through a common signaling pathway that is necessary for normal tubulogenesis. SUBUNIT: Interacts with PKD1. PKD1 requires the presence of PKD2 for stable expression. Interacts with CD2AP. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Strongly expressed in ovary, fetal and adult kidney, testis, and small intestine. Not detected in peripheral leukocytes. DISEASE: Defects in PKD2 are the cause of polycystic kidney disease autosomal dominant type 2 (ADPKD2). ADPKD2 represents approximately 15% of the cases of ADPKD, a common genetic disease affecting about 1:400 to 1:1000 individuals. ADPKD is characterized by progressive formation and enlargement of cysts in both kidneys, typically leading to end-stage renal disease in adult life. Cysts also occurs in the liver and other organs. ADPKD2 is clinically milder than ADPKD1 but it has a deleterious impact on overall life expectancy. Also known as: PKD2, Polycystin-2, Autosomal dominant polycystic kidney disease type II protein, Polycystwin, R48321.

Specificity: Appears to be specific for PKD2.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Mochizuki, Wu, Hayashi et al.: "PKD2, a gene for polycystic kidney disease that encodes an integral membrane protein." in: Science (New York, N.Y.), Vol. 272, Issue 5266, pp. 1339-42, 1996 (PubMed).

Schneider, Rodriguez, Nomura et al.: "A gene similar to PKD1 maps to chromosome 4q22: a candidate gene for PKD2." in: Genomics, Vol. 38, Issue 1, pp. 1-4, 1997 (PubMed).

Hayashi, Mochizuki, Reynolds et al.: "Characterization of the exon structure of the polycystic kidney disease 2 gene (PKD2)." in: Genomics, Vol. 44, Issue 1, pp. 131-6, 1997 (PubMed).

Veldhuisen, Saris, de Haij et al.: "A spectrum of mutations in the second gene for autosomal dominant polycystic kidney disease (PKD2)." in: American journal of human genetics, Vol. 61, Issue 3, pp. 547-55, 1997 (PubMed).

Torra, Viribay, Telleruia et al.: "Seven novel mutations of the PKD2 gene in families with autosomal dominant polycystic kidney disease." in: Kidney international, Vol. 56, Issue 1, pp. 28-33, 1999 (PubMed).

Watnick, He, Wang et al.: "Mutations of PKD1 in ADPKD2 cysts suggest a pathogenic effect of trans-heterozygous mutations." in: Nature genetics, Vol. 25, Issue 2, pp. 143-4, 2000 (PubMed).

Lehtonen, Ora, Olkkonen et al.: "In vivo interaction of the adapter protein CD2-associated protein with the type 2 polycystic kidney disease protein, polycystin-2." in: The Journal of biological chemistry, Vol. 275, Issue 42, pp. 32888-93, 2000 (PubMed).

Stayner, Zhou: "Polycystin channels and kidney disease." in: Trends in pharmacological sciences, Vol. 22, Issue 11, pp. 543-6, 2001 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Molina, Horn, Tang et al.: "Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 7, pp. 2199-204, 2007 (PubMed).