polycystic kidney disease 2-like 1 protein (PK2L1, PKD2L1) (1st Cytoplasmic Domain) antibody

Antigen: polycystic kidney disease 2-like 1 protein (PK2L1, PKD2L1)

Binding Site: 1st Cytoplasmic Domain

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350746

Additional Information

UniProt: Q9P0L9

Immunogen: A synthetic peptide from the 1t cytoplasmic loop of human Polycystic kidney disease 2-like 1 protein (PK2L1, PKD2L1, PKD2L, PKDL, Polycystin-L) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: This gene encodes a member of the polycystin protein family. The encoded protein contains multipletransmembrane domains, and cytoplasmic N- and C-termini. The protein may be an integral membrane protein involved in cell-cell/matrix interactions. This protein functions as a calcium-regulated nonselective cation channel. Alternative splice variants have been described but their full length sequences have not been determined FUNCTION: May function as a subunit of an ion channel and act as a transducer of calcium-mediated signaling. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed in adult heart, skeletal muscle, brain, spleen, testis, retina and liver. According to Ref.1 expressed at high levels in fetal tissues, including kidney and liver, and down-regulated in adult tissues. It has been found to be expressed in fetal brain, but not expressed in fetal lung, liver or kidney. Isoform 4 appears to be expressed only in transformed lymphoblasts. Also known as: PK2L1, PKD2L1, PKD2L, PKDL, Polycystin-L.

Specificity: Appears to be specific for PKD2L1.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Nomura, Turco, Pei et al.: "Identification of PKDL, a novel polycystic kidney disease 2-like gene whose murine homologue is deleted in mice with kidney and retinal defects." in: The Journal of biological chemistry, Vol. 273, Issue 40, pp. 25967-73, 1998 (PubMed).

Wu, Hayashi, Park et al.: "Identification of PKD2L, a human PKD2-related gene: tissue-specific expression and mapping to chromosome 10q25." in: Genomics, Vol. 54, Issue 3, pp. 564-8, 1999 (PubMed).

Chen, Vassilev, Basora et al.: "Polycystin-L is a calcium-regulated cation channel permeable to calcium ions." in: Nature, Vol. 401, Issue 6751, pp. 383-6, 1999 (PubMed).

Veldhuisen, Spruit, Dauwerse et al.: "Genes homologous to the autosomal dominant polycystic kidney disease genes (PKD1 and PKD2)." in: European journal of human genetics : EJHG, Vol. 7, Issue 8, pp. 860-72, 2000 (PubMed).

Guo, Chen, Basora et al.: "The human polycystic kidney disease 2-like (PKDL) gene: exon/intron structure and evidence for a novel splicing mechanism." in: Mammalian genome : official journal of the International Mammalian Genome Society, Vol. 11, Issue 1, pp. 46-50, 2000 (PubMed).

Stayner, Zhou: "Polycystin channels and kidney disease." in: Trends in pharmacological sciences, Vol. 22, Issue 11, pp. 543-6, 2001 (PubMed).