HCN2 antibody (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 2) (1st Cytoplasmic Domain)

Details for Product anti-HCN2 Antibody No. ABIN350759, Supplier: Log in to see
Antigen
  • HCN2
  • BCNG-2
  • BCNG2
  • HAC-1
  • HAC1
  • trls
  • hyperpolarization activated cyclic nucleotide-gated potassium channel 2
  • hyperpolarization-activated, cyclic nucleotide-gated K+ 2
  • LOC428335
  • HCN2
  • Hcn2
Epitope
1st Cytoplasmic Domain
36
7
4
2
2
1
1
1
1
1
1
1
Reactivity
Human
56
55
47
2
Host
Rabbit
39
30
Clonality
Polyclonal
Conjugate
This HCN2 antibody is un-conjugated
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
67
45
38
37
36
18
17
3
2
1
1
Options
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Immunogen A synthetic peptide from the 1t cytoplasmic domain of human potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 (HCN2) conjugated to an immunogenic carrier protein was used as the immunogen.
Specificity Specific for HCN2.
Purification Whole serum
Alternative Name HCN2 (HCN2 Antibody Abstract)
Background HCN2:
Function: Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH, acidic pH shifts the activation to more negative voltages.
Subunit: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits. Heteromultimer with HCN1. Interacts with KCNE2.
Subcellular location: Membrane, Multi-pass membrane protein.
Tissue specificity: Highly expressed in neonatal and adult ventricle and in brain. Highly expressed in the pyramidal layer in hippocampus, in anterior dorsal nucleus in thalamus, in the mammillary nucleus in hypothalamus, in red nucleus, in trigeminal mesencephalic, spinal and principal nuclei, in cochlear and trapezoid nuclei and in the dorsal tegemental nucleus. Also known as: HCN2 (Brain cyclic nucleotide-gated channel 2, BCNG-2, BCNG2, HAC-1).
Research Area Cardiovascular, Contractility
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Grimwood, Gordon, Olsen, Terry, Schmutz, Lamerdin, Hellsten, Goodstein, Couronne, Tran-Gyamfi, Aerts, Altherr, Ashworth, Bajorek, Black, Branscomb, Caenepeel, Carrano, Caoile, Chan, Christensen et al.: "The DNA sequence and biology of human chromosome 19. ..." in: Nature, Vol. 428, Issue 6982, pp. 529-35, 2004 (PubMed).

Ludwig, Zong, Stieber, Hullin, Hofmann, Biel: "Two pacemaker channels from human heart with profoundly different activation kinetics." in: The EMBO journal, Vol. 18, Issue 9, pp. 2323-9, 1999 (PubMed).

Vaccari, Moroni, Rocchi, Gorza, Bianchi, Beltrame, DiFrancesco: "The human gene coding for HCN2, a pacemaker channel of the heart." in: Biochimica et biophysica acta, Vol. 1446, Issue 3, pp. 419-25, 1999 (PubMed).

Santoro, Liu, Yao, Bartsch, Kandel, Siegelbaum, Tibbs: "Identification of a gene encoding a hyperpolarization-activated pacemaker channel of brain." in: Cell, Vol. 93, Issue 5, pp. 717-29, 1998 (PubMed).