Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 2 (HCN2) (1st Cytoplasmic Domain) antibody

Antigen: Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 2 (HCN2)

Synonyms: BCNG2, HAC-1, BCNG-2, HCN2, wu:fj61d07, si:dkey-28h18.1

Binding Site: 1st Cytoplasmic Domain

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350759

Additional Information

Alternative name: potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 2 (HCN2)

UniProt: Q9UL51

Immunogen: A synthetic peptide from the 1t cytoplasmic domain of human potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 (HCN2) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: HCN2: FUNCTION: Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages. SUBUNIT: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits. Heteromultimer with HCN1. Interacts with KCNE2. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Highly expressed in neonatal and adult ventricle and in brain. Highly expressed in the pyramidal layer in hippocampus, in anterior dorsal nucleus in thalamus, in the mammillary nucleus in hypothalamus, in red nucleus, in trigeminal mesencephalic, spinal and principal nuclei, in cochlear and trapezoid nuclei and in the dorsal tegemental nucleus. Also known as: HCN2 (Brain cyclic nucleotide-gated channel 2, BCNG-2, BCNG2, HAC-1).

Specificity: Appears to be specific for HCN2.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Santoro, Liu, Yao et al.: "Identification of a gene encoding a hyperpolarization-activated pacemaker channel of brain." in: Cell, Vol. 93, Issue 5, pp. 717-29, 1998 (PubMed).

Ludwig, Zong, Stieber et al.: "Two pacemaker channels from human heart with profoundly different activation kinetics." in: The EMBO journal, Vol. 18, Issue 9, pp. 2323-9, 1999 (PubMed).

Vaccari, Moroni, Rocchi et al.: "The human gene coding for HCN2, a pacemaker channel of the heart." in: Biochimica et biophysica acta, Vol. 1446, Issue 3, pp. 419-25, 1999 (PubMed).

Grimwood, Gordon, Olsen et al.: "The DNA sequence and biology of human chromosome 19." in: Nature, Vol. 428, Issue 6982, pp. 529-35, 2004 (PubMed).