Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 1 (HCN1) antibody

Antigen: Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 1 (HCN1)

Synonyms: BCNG1, HAC-2, BCNG-1, HCN1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350760

Additional Information

Alternative name: potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 (HCN1)

UniProt: O60741

Immunogen: A synthetic peptide from human potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 (HCN1) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. May mediate responses to sour stimuli. SUBUNIT: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits. Heteromultimer with HCN2. Interacts with KCNE2. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Detected in brain, in particular in amygdala and hippocampus, while expression in caudate nucleus, corpus callosum, substantia nigra, subthalamic nucleus and thalamus is very low or not detectable. Detected at very low levels in muscle and pancreas. DOMAIN: The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. Also known as: Brain cyclic nucleotide-gated channel 1, BCNG-1, BCNG1, HAC-2.

Specificity: Appears to be specific for HCN1.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Santoro, Grant, Bartsch et al.: "Interactive cloning with the SH3 domain of N-src identifies a new brain specific ion channel protein, with homology to eag and cyclic nucleotide-gated channels." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 26, pp. 14815-20, 1998 (PubMed).

Santoro, Liu, Yao et al.: "Identification of a gene encoding a hyperpolarization-activated pacemaker channel of brain." in: Cell, Vol. 93, Issue 5, pp. 717-29, 1998 (PubMed).

Schmutz, Martin, Terry et al.: "The DNA sequence and comparative analysis of human chromosome 5." in: Nature, Vol. 431, Issue 7006, pp. 268-74, 2004 (PubMed).