Search, Find and Buy Antibodies, ELISA Kits and Proteins.
Loading Loading
Order hotline:
phone +1 404 474 4654
fax +1 888 205 9894 (TF)
Details for Product No. ABIN350762

Potassium/sodium Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel 2 and 4 (HCN2, HCN4) (1st Cytoplasmic Domain) antibody

Request
Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Epitope
1st Cytoplasmic Domain
Host
Rabbit
Clonality Polyclonal
Application
Immunohistochemistry (IHC), Western Blotting (WB)
Pubmed 6 references available
Catalog no. ABIN350762
Quantity 100 µL
Price
454.67 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 7 to 8 Business Days
Immunogen A synthetic peptide from the 1t cytoplasmic domain of human potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 and 4 (HCN2, HCN4) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat and mouse.
Specificity Appears to be specific for HCN2, HCN4.
Purity whole serum
Background HCN2:
Function: Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH, acidic pH shifts the activation to more negative voltages.
Subunit: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits. Heteromultimer with HCN1. Interacts with KCNE2.
Subcellular location: Membrane, Multi-pass membrane protein.
Tissue specificity: Highly expressed in neonatal and adult ventricle and in brain. Highly expressed in the pyramidal layer in hippocampus, in anterior dorsal nucleus in thalamus, in the mammillary nucleus in hypothalamus, in red nucleus, in trigeminal mesencephalic, spinal and principal nuclei, in cochlear and trapezoid nuclei and in the dorsal tegemental nucleus. INDUCTION: By thyroid hormones in hypothyroid animals. DOMAIN: The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. HCN4:
Function: Hyperpolarization-activated ion channel with very slow activation and inactivation exhibiting weak selectivity for potassium over sodium ions. May contribute to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP. May mediate responses to sour stimuli.
Subunit: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits.
Subcellular location: Membrane, Multi-pass membrane protein.
Tissue specificity: Highly expressed in thalamus, testis and in heart, both in ventricle and atrium. Detected at much lower levels in amygdala, substantia nigra, cerebellum and hippocampus. Also known as: HCN2 (Brain cyclic nucleotide-gated channel 2, BCNG-2, BCNG2, HAC-1), HCN4.
UniProt Q9UL51
Application Notes IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Storage -20 °C
Ludwig, Zong, Stieber et al.: "Two pacemaker channels from human heart with profoundly different activation kinetics." in: The EMBO journal, Vol. 18, Issue 9, pp. 2323-9, 1999 (PubMed).

Shi, Wymore, Yu et al.: "Distribution and prevalence of hyperpolarization-activated cation channel (HCN) mRNA expression in cardiac tissues." in: Circulation research, Vol. 85, Issue 1, pp. e1-6, 1999 (PubMed).

Pachucki, Burmeister, Larsen: "Thyroid hormone regulates hyperpolarization-activated cyclic nucleotide-gated channel (HCN2) mRNA in the rat heart." in: Circulation research, Vol. 85, Issue 6, pp. 498-503, 1999 (PubMed).

Monteggia, Eisch, Tang et al.: "Cloning and localization of the hyperpolarization-activated cyclic nucleotide-gated channel family in rat brain." in: Brain research. Molecular brain research, Vol. 81, Issue 1-2, pp. 129-39, 2000 (PubMed).

Ueda, Nakamura, Hayashi et al.: "Functional characterization of a trafficking-defective HCN4 mutation, D553N, associated with cardiac arrhythmia." in: The Journal of biological chemistry, Vol. 279, Issue 26, pp. 27194-8, 2004 (PubMed).

Kimura, Kitano, Nakajima et al.: "Hyperpolarization-activated, cyclic nucleotide-gated HCN2 cation channel forms a protein assembly with multiple neuronal scaffold proteins in distinct modes of protein-protein interaction." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 9, Issue 7, pp. 631-40, 2004 (PubMed).

Beacon