Proteinase 3 (PRTN3) (Internal Region) antibody

Details for Product No. ABIN350765
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Antigen
Synonyms ela2, MGC132250, ACPA, AGP7, C-ANCA, CANCA, MBN, MBT, NP-4, NP4, P29, PR-3, PR3, mPR3
Epitope
Internal Region
(12), (9), (6), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(93), (17), (16), (1), (1), (1), (1)
Host
Rabbit
(51), (47), (3), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(77), (60), (12), (12), (11), (10), (8), (8), (6), (3), (2), (2)
Pubmed 5 references available
Quantity 100 µL
Options
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Catalog No. ABIN350765
454.67 $
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Immunogen A synthetic peptide from the internal region of human Pr3 (Leukocyte proteinase 3, AGP7, Myeloblastin, Wegener autoantigen) conjugated to an immunogenic carrier protein was used as the immunogen.
Specificity Specific for Pr3.
Purification Whole serum
Alternative Name Pr3
Background Function: Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to s.
Catalytic activity: Hydrolysis of proteins, including elastin, by preferential cleavage: -Ala-|-Xaa- > -Val-|-Xaa-. Also known as: Leukocyte proteinase 3, AGP7, Myeloblastin, Wegener autoantigen, PR-3, P29, C-ANCA antigen, Neutrophil proteinase 4, MBN, PRTN3, NP-4.
Application Notes A dilution of 1: 200 to 1: 1000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Supplier Images
anti-Proteinase 3 (PRTN3) (Internal Region) antibody WB on human PMN (peripheral blood mononuclear cells isolated from buffycoat. Denatured, reduced) using Rabbit antibody to internal region of Pr3 (Wegener autoantigen): whole serum (ABIN350765) at 1: 500 dilution. Blocked with 1% LFDM for 15 minutes at room temperature with shake, primary antibody incubated for 15 minutes at room temperature, washed 3 times with PBS (1x), 5 minutes each. Secondary antibody was also incubated for 15 minutes at room temperature.
General Labbaye, Musette, Cayre: "Wegener autoantigen and myeloblastin are encoded by a single mRNA." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 20, pp. 9253-6, 1991 (PubMed).

Jenne, Tschopp, Luedemann et al.: "Wegener's autoantigen decoded." in: Nature, Vol. 346, Issue 6284, pp. 520, 1990 (PubMed).

Duke-Cohan, Morimoto, Rocker et al.: "A novel form of dipeptidylpeptidase IV found in human serum. Isolation, characterization, and comparison with T lymphocyte membrane dipeptidylpeptidase IV (CD26)." in: The Journal of biological chemistry, Vol. 270, Issue 23, pp. 14107-14, 1995 (PubMed).

Fujinaga, Chernaia, Halenbeck et al.: "The crystal structure of PR3, a neutrophil serine proteinase antigen of Wegener's granulomatosis antibodies." in: Journal of molecular biology, Vol. 261, Issue 2, pp. 267-78, 1996 (PubMed).

Grimwood, Gordon, Olsen et al.: "The DNA sequence and biology of human chromosome 19." in: Nature, Vol. 428, Issue 6982, pp. 529-35, 2004 (PubMed).

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