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Proteinase 3 (PRTN3) (N-Term) antibody

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Antigen
  • ACPA
  • AGP7
  • C-ANCA
  • CANCA
  • ela2
  • MBN
  • MBT
  • MGC132250
  • mPR3
  • NP-4
  • NP4
  • P29
  • PR-3
  • PR3
Epitope
N-Term
22
17
13
11
8
7
6
5
5
4
2
2
2
2
2
Reactivity
Human
170
7
6
1
1
1
Host
Rabbit
103
63
14
3
2
Clonality
Polyclonal
Conjugate
Un-conjugated
15
11
6
6
5
5
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
155
121
23
14
14
12
9
6
6
6
4
3
2
1
1
Supplier
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Immunogen A synthetic peptide from the n-terminal region of human Pr3 conjugated to an immunogenic carrier protein was used as the antigen.
Isotype IgG
Specificity Specific for Pr3.
Alternative Name Pr3 (PRTN3 Antibody Abstract)
Background Function: Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to s.
Catalytic activity: Hydrolysis of proteins, including elastin, by preferential cleavage: -Ala-|-Xaa- > -Val-|-Xaa-. Also known as: Leukocyte proteinase 3, AGP7, Myeloblastin, Wegener autoantigen, PR-3, P29, C-ANCA antigen, Neutrophil proteinase 4, MBN, PRTN3, NP-4.
Application Notes A concentration of 10-50 is recommended.
The optimal concentration should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Grimwood, Gordon, Olsen, Terry, Schmutz, Lamerdin, Hellsten, Goodstein, Couronne, Tran-Gyamfi, Aerts, Altherr, Ashworth, Bajorek, Black, Branscomb, Caenepeel, Carrano, Caoile, Chan, Christensen et al.: "The DNA sequence and biology of human chromosome 19. ..." in: Nature, Vol. 428, Issue 6982, pp. 529-35, 2004 (PubMed).

Fujinaga, Chernaia, Halenbeck, Koths, James: "The crystal structure of PR3, a neutrophil serine proteinase antigen of Wegener's granulomatosis antibodies." in: Journal of molecular biology, Vol. 261, Issue 2, pp. 267-78, 1996 (PubMed).

Duke-Cohan, Morimoto, Rocker, Schlossman: "A novel form of dipeptidylpeptidase IV found in human serum. Isolation, characterization, and comparison with T lymphocyte membrane dipeptidylpeptidase IV (CD26)." in: The Journal of biological chemistry, Vol. 270, Issue 23, pp. 14107-14, 1995 (PubMed).

Labbaye, Musette, Cayre: "Wegener autoantigen and myeloblastin are encoded by a single mRNA." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 20, pp. 9253-6, 1991 (PubMed).

Jenne, Tschopp, Luedemann, Utecht, Gross: "Wegener's autoantigen decoded." in: Nature, Vol. 346, Issue 6284, pp. 520, 1990 (PubMed).