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Pro BDNF antibody

Details for Product No. ABIN350770, Supplier: Log in to see
Antigen
Reactivity
Rat (Rattus), Mouse (Murine)
3
3
3
Host
Sheep
5
1
Clonality
Polyclonal
Application
Immunohistochemistry (IHC), Western Blotting (WB)
6
6
Supplier
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Immunogen A synthetic peptide from rat proBDNF conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse and zebrafish and shares 96% identity with human sequence.
Isotype IgG
Specificity Specific for proBDNF.
Background Function: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
Subunit: Monomers and homodimers. Binds to NTRK2/TRKB.
Subcellular location: Secreted. Also known as: Brain-derived neurotrophic factor precursor, proBDNF, pro BDNF.
Application Notes A concentration of 10-50 µg/ml is recommended.
The optimal concentration should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Aid, Kazantseva, Piirsoo, Palm, Timmusk: "Mouse and rat BDNF gene structure and expression revisited." in: Journal of neuroscience research, Vol. 85, Issue 3, pp. 525-35, 2007

Liu, Lu, Zhu, Gong, Shaham, Uhl: "Rodent BDNF genes, novel promoters, novel splice variants, and regulation by cocaine." in: Brain research, Vol. 1067, Issue 1, pp. 1-12, 2006

Timmusk, Palm, Metsis, Reintam, Paalme, Saarma, Persson: "Multiple promoters direct tissue-specific expression of the rat BDNF gene." in: Neuron, Vol. 10, Issue 3, pp. 475-89, 1993

Ohara, Gahara, Teraoka, Kitamura: "A rat brain-derived neurotrophic factor-encoding gene generates multiple transcripts through alternative use of 5' exons and polyadenylation sites." in: Gene, Vol. 121, Issue 2, pp. 383-6, 1992

Yancopoulos, Maisonpierre, Ip, Aldrich, Belluscio, Boulton, Cobb, Squinto, Furth: "Neurotrophic factors, their receptors, and the signal transduction pathways they activate." in: Cold Spring Harbor symposia on quantitative biology, Vol. 55, pp. 371-9, 1992

Maisonpierre, Le Beau, Espinosa, Ip, Belluscio, de la Monte, Squinto, Furth, Yancopoulos: "Human and rat brain-derived neurotrophic factor and neurotrophin-3: gene structures, distributions, and chromosomal localizations." in: Genomics, Vol. 10, Issue 3, pp. 558-68, 1991

Hallboeoek, Ibuaunez, Persson: "Evolutionary studies of the nerve growth factor family reveal a novel member abundantly expressed in Xenopus ovary." in: Neuron, Vol. 6, Issue 5, pp. 845-58, 1991