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PCNA antibody (Proliferating Cell Nuclear Antigen) (C-Term)

Details for Product anti-PCNA Antibody No. ABIN350771, Supplier: Log in to see
Antigen
  • 53/13
  • AO090003000846
  • cb16
  • CG9193
  • Dmel\\CG9193
  • DmPCNA
  • dPCNA
  • fb36g03
  • l(2)02448
  • l(2)56F
  • l(2)56Fa
  • l(2)s1534
  • MUS209
  • Mus209
  • Pcna
  • pcna
  • PCNA
  • pcna-A
  • Pcna/cyclin
  • PCNA/mus209
  • PCNAR
  • wu:fa28e03
  • wu:fb36g03
Alternatives
anti-Mouse (Murine) PCNA antibody for Immunohistochemistry
Epitope
C-Term
34
32
27
15
14
14
13
8
7
7
7
5
5
5
4
4
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus), Mouse (Murine)
542
232
209
90
62
57
51
50
41
32
26
24
24
17
9
7
7
7
4
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
Host
Rabbit
407
193
8
3
Clonality
Polyclonal
Conjugate
This PCNA antibody is un-conjugated
22
22
12
11
11
10
9
9
8
8
8
8
8
8
8
8
7
7
5
2
2
2
2
2
2
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
477
225
181
169
141
135
127
87
62
30
7
7
7
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Supplier
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Immunogen A synthetic peptide from the c-terminal region of rat Proliferating cell nuclear antigen (PCNA) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in human and rat.
Specificity Specific for PCNA.
Purification Whole serum
Alternative Name PCNA (PCNA Antibody Abstract)
Background Function: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Involvement in disease: Antibodies are present in sera from patients with systemic lupus erythematosus. Also known as: Cyclin, DNA polymerase delta auxiliary protein, Polymerase delta accessory protein, Proliferating Cell Nuclear Antigen.
Research Area Cancer, Cell Cycle, Chromatin Binding Proteins, DNA/RNA, Proliferation Markers
Pathways Telomere Maintenance, DNA Damage Repair
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Unk, Hajdú, Fátyol, Szakál, Blastyák, Bermudez, Hurwitz, Prakash, Prakash, Haracska: "Human SHPRH is a ubiquitin ligase for Mms2-Ubc13-dependent polyubiquitylation of proliferating cell nuclear antigen." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 48, pp. 18107-12, 2006 (PubMed).

Sakurai, Kitano, Yamaguchi, Hamada, Okada, Fukuda, Uchida, Ohtsuka, Morioka, Hakoshima: "Structural basis for recruitment of human flap endonuclease 1 to PCNA." in: The EMBO journal, Vol. 24, Issue 4, pp. 683-93, 2005 (PubMed).

Deloukas, Matthews, Ashurst, Burton, Gilbert, Jones, Stavrides, Almeida, Babbage, Bagguley, Bailey, Barlow, Bates, Beard, Beare, Beasley, Bird, Blakey, Bridgeman, Brown, Buck, Burrill, Butler, Carder et al.: "The DNA sequence and comparative analysis of human chromosome 20. ..." in: Nature, Vol. 414, Issue 6866, pp. 865-71, 2002 (PubMed).

Almendral, Huebsch, Blundell, Macdonald-Bravo, Bravo: "Cloning and sequence of the human nuclear protein cyclin: homology with DNA-binding proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 6, pp. 1575-9, 1987 (PubMed).