Proliferating Cell Nuclear Antigen (PCNA) (C-Term) antibody

Antigen: Proliferating Cell Nuclear Antigen (PCNA)

Synonyms: MGC8367, PCNAR, Pcna/cyclin, pcna

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350771

Additional Information

Alternative name: Prolifeing cell nuclear antigen (PCNA) (c-terminal region)

Swiss-Prot: P04961

Immunogen: A synthetic peptide from the c-terminal region of rat Proliferating cell nuclear antigen (PCNA) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in human and rat.

Format: Lyophilized

Description: Function: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Involvement in disease: Antibodies are present in sera from patients with systemic lupus erythematosus. Also known as: Cyclin, DNA polymerase delta auxiliary protein, Polymerase delta accessory protein, Proliferating Cell Nuclear Antigen.

Specificity: Appears to be specific for PCNA.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Almendral, Huebsch, Blundell et al.: "Cloning and sequence of the human nuclear protein cyclin: homology with DNA-binding proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 6, pp. 1575-9, 1987 (PubMed).

Deloukas, Matthews, Ashurst et al.: "The DNA sequence and comparative analysis of human chromosome 20." in: Nature, Vol. 414, Issue 6866, pp. 865-71, 2002 (PubMed).

Sakurai, Kitano, Yamaguchi et al.: "Structural basis for recruitment of human flap endonuclease 1 to PCNA." in: The EMBO journal, Vol. 24, Issue 4, pp. 683-93, 2005 (PubMed).

Unk, Hajduu, Fuatyol et al.: "Human SHPRH is a ubiquitin ligase for Mms2-Ubc13-dependent polyubiquitylation of proliferating cell nuclear antigen." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 48, pp. 18107-12, 2006 (PubMed).