Rabphilin 3A Homolog (Mouse) (RPH3A) antibody

Antigen: Rabphilin 3A Homolog (Mouse) (RPH3A)

Synonyms: KIAA0985, AU022689, AW108370, 2900002P20Rik, DmelCG11556, CG11556

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350777

Additional Information

Alternative name: Rabphilin-3A (Exophilin-1, Rph3a)

Swiss-Prot: P47709

Immunogen: A synthetic peptide as a part of rat, mouse Rabphilin-3A (Exophilin-1, Rph3a) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Protein transport. Probably involved with Ras-related protein Rab-3A in synaptic vesicle traffic and/or synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal. SUBUNIT: Monomer. SUBCELLULAR LOCATION: Cell junction, synapse. TISSUE SPECIFICITY: Specifically expressed in brain. Also known as: Exophilin-1, Rph3a, rabphilin 3A homolog.

Specificity: Appears to be specific for Rabphilin-3A.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

IF on 1 day- old mouse cortical cell culture using Rabbit antibody to rat, mouse Rabphilin-3A (Exophilin-1, Rph3a): whole serum (ABIN350777) at 1: 2000 dilution. DAPI counter stained.

IF on 1 day- old mouse cortical cell culture using Rabbit antibody to rat, mouse Rabphilin-3A (Exophilin-1, Rph3a): whole serum (ABIN350777) at 1: 2000 dilution. DAPI counter stained.

Publications

Publications: Inagaki, Mizuta, Seino: "Cloning of a mouse Rabphilin-3A expressed in hormone-secreting cells." in: Journal of biochemistry, Vol. 116, Issue 2, pp. 239-42, 1995 (PubMed).

Li, Takei, Geppert et al.: "Synaptic targeting of rabphilin-3A, a synaptic vesicle Ca2+/phospholipid-binding protein, depends on rab3A/3C." in: Neuron, Vol. 13, Issue 4, pp. 885-98, 1994 (PubMed).

Munton, Tweedie-Cullen, Livingstone-Zatchej et al.: "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 2, pp. 283-93, 2007 (PubMed).