Ras-Related C3 Botulinum Toxin Substrate 1 (Rho Family, Small GTP Binding Protein Rac1) (RAC1) antibody

Antigen: Ras-Related C3 Botulinum Toxin Substrate 1 (Rho Family, Small GTP Binding Protein Rac1) (RAC1)

Synonyms: MIG5, TC-25, p21-Rac1, MGC111543

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN350778

Additional Information

Alternative name: RAC1, RAC2 (Ras-like protein TC25, p21-Rac2)

UniProt: P63000

Immunogen: A synthetic peptide from of human RAC1, RAC2 (Ras-like protein TC25, p21-Rac2) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat, mouse, guinea pig, bovine, xenopus, dog, zebrafish and chicken.

Format: Lyophilized

Description: FUNCTION: Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. ENZYME REGULATION: Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase. SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side. Melanosome. Note: Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. RAC1: TISSUE SPECIFICITY: Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. The expression of isoform B is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues. RAC2: TISSUE SPECIFICITY: Hematopoietic specific. DISEASE: Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome. Also known as: RAC1 is also known as: p21-Rac1, Ras-like protein TC25, Cell migration-inducing gene 5 protein; RAC2 is also known as: p21-Rac2, Small G protein, GX.

Specificity: Appears to be specific for RAC1 and RAC2.

Application Details

Application Notes: IHC. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

IHC on rat ligated sciatic nerve using Rabbit antibody to RAC1, RAC2 (Ras-like protein TC25, p21-Rac2): whole serum (ABIN350778) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

IHC on rat sciatic nerve using Rabbit antibody to RAC1, RAC2 (Ras-like protein TC25, p21-Rac2): whole serum (ABIN350778) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

Publications

Jordan, Brazåo, Boavida et al.: "Cloning of a novel human Rac1b splice variant with increased expression in colorectal tumors." in: Oncogene, Vol. 18, Issue 48, pp. 6835-9, 2000 (PubMed).

Ambruso, Knall, Abell et al.: "Human neutrophil immunodeficiency syndrome is associated with an inhibitory Rac2 mutation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 9, pp. 4654-9, 2000 (PubMed).

Worthylake, Rossman, Sondek: "Crystal structure of Rac1 in complex with the guanine nucleotide exchange region of Tiam1." in: Nature, Vol. 408, Issue 6813, pp. 682-8, 2000 (PubMed).

Hillier, Fulton, Fulton et al.: "The DNA sequence of human chromosome 7." in: Nature, Vol. 424, Issue 6945, pp. 157-64, 2003 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).

Prehna, Ivanov, Bliska et al.: "Yersinia virulence depends on mimicry of host Rho-family nucleotide dissociation inhibitors." in: Cell, Vol. 126, Issue 5, pp. 869-80, 2006 (PubMed).

Sjöblom, Jones, Wood et al.: "The consensus coding sequences of human breast and colorectal cancers." in: Science (New York, N.Y.), Vol. 314, Issue 5797, pp. 268-74, 2006 (PubMed).

Jezyk, Snyder, Gershberg et al.: "Crystal structure of Rac1 bound to its effector phospholipase C-beta2." in: Nature structural & molecular biology, Vol. 13, Issue 12, pp. 1135-40, 2006 (PubMed).