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RAB5B, Member RAS Oncogene Family (RAB5B) (AA 175-212) antibody

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Antigen
  • zgc:76978
  • C030027M18Rik
Epitope
AA 175-212
13
10
10
8
3
2
Reactivity
Human, Mouse (Murine)
31
14
9
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Host
Rabbit
30
2
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
2
2
2
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
28
18
2
2
2
1
Supplier
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Immunogen A synthetic peptide from aa region 175-212 of human RAB5B conjugated to blue carrier protein has been used as the antigen. The antigen is homologous in mouse and shares 98% identity with rat.
Specificity Specific for RAB5B.
Purification Whole serum
Alternative Name Rab5B (RAB5B Antibody Abstract)
Background Function: Protein transport. Probably involved in vesicular traffic.
Enzyme regulation: Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP.
Subcellular location: Cell membrane, Lipid-anchor, Cytoplasmic side. Early endosome membrane, Lipid-anchor. Melanosome. Note: Enriched in stage I melanosomes. Also known as: RAB5B.
Pathways
Application Notes Use at a dilution of 1:300 to 1: 2000.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Chi, Valencia, Hu et al.: "Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes." in: Journal of proteome research, Vol. 5, Issue 11, pp. 3135-44, 2006 (PubMed).

Saito, Murai, Kajiho et al.: "A novel binding protein composed of homophilic tetramer exhibits unique properties for the small GTPase Rab5." in: The Journal of biological chemistry, Vol. 277, Issue 5, pp. 3412-8, 2002 (PubMed).

Callaghan, Nixon, Bucci et al.: "Direct interaction of EEA1 with Rab5b." in: European journal of biochemistry / FEBS, Vol. 265, Issue 1, pp. 361-6, 1999 (PubMed).

Wilson, Wilson: "Identification and subcellular localization of human rab5b, a new member of the ras-related superfamily of GTPases." in: The Journal of clinical investigation, Vol. 89, Issue 3, pp. 996-1005, 1992 (PubMed).