RAB5B, Member RAS Oncogene Family (RAB5B) (Internal Region) antibody

Antigen: RAB5B, Member RAS Oncogene Family (RAB5B)

Synonyms: MGC76978, zgc:76978

Binding Site: Internal Region

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350783

Additional Information

Alternative name: Ras-related protein Rab-5B (RAB5B)

UniProt: P61020

Immunogen: A synthetic peptide from the internal part of human Ras-related protein Rab-5B (RAB5B) conjugated to an immunogenic carrier protein has been used as the immunogen. The antigen is homologous in mouse and shares 98% identity with rat.

Format: Lyophilized

Description: FUNCTION: Protein transport. Probably involved in vesicular traffic. ENZYME REGULATION: Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP. SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side. Early endosome membrane; Lipid-anchor. Melanosome. Note: Enriched in stage I melanosomes. Also known as: RAB5B.

Specificity: Appears to be specific for RAB5B.

Application Details

Application Notes: IHC, WB. Use at a dilution of 1:300 to 1: 2000. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Wilson, Wilson: "Identification and subcellular localization of human rab5b, a new member of the ras-related superfamily of GTPases." in: The Journal of clinical investigation, Vol. 89, Issue 3, pp. 996-1005, 1992 (PubMed).

Callaghan, Nixon, Bucci et al.: "Direct interaction of EEA1 with Rab5b." in: European journal of biochemistry / FEBS, Vol. 265, Issue 1, pp. 361-6, 1999 (PubMed).

Saito, Murai, Kajiho et al.: "A novel binding protein composed of homophilic tetramer exhibits unique properties for the small GTPase Rab5." in: The Journal of biological chemistry, Vol. 277, Issue 5, pp. 3412-8, 2002 (PubMed).

Chi, Valencia, Hu et al.: "Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes." in: Journal of proteome research, Vol. 5, Issue 11, pp. 3135-44, 2006 (PubMed).