Ras-related Protein Ral-A (rala) antibody

Antigen: Ras-related Protein Ral-A (rala)

Synonyms: RAL, MGC48949

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350787

Additional Information

Alternative name: Ras-related protein Ral-A (Rala, Ral, Ral-a)

Swiss-Prot: P11233

Immunogen: A synthetic peptide from human Ras-related protein Ral-A (Rala, Ral, Ral-a) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat and mouse.

Format: Lyophilized

Description: The product of this gene belongs to the small GTPase superfamily, Ras family of proteins. GTP-binding proteins mediate thetransmembrane signaling initiated by the occupancy of certain cell surface receptors. This gene encodes a low molecular mass ras-like GTP-binding protein that shares about 50% similarity with other ras proteins. SUBUNIT: Interacts with RALBP1 via its effector domain. Interacts with EXOC8 and EXOC2. EXOC2 and EXOC8 have overlapping binding sites and compete for RALA binding. Interacts with Clostridium exoenzyme C3. Interacts with RALGPS1. SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side. Also known as: Rala, Ral, Ral-a, MGC48949.

Specificity: Appears to be specific for Ral-A.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

IHC on rat brain (cortex) using Rabbit antibody to Ras-related protein Ral-A (Rala, Ral, Ral-a): whole serum (ABIN350787) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

IHC on rat septum using Rabbit antibody to Ras-related protein Ral-A (Rala, Ral, Ral-a): whole serum (ABIN350787) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

IHC on rat spinal cord (dorsal horn) using Rabbit antibody to Ras-related protein Ral-A (Rala, Ral, Ral-a): whole serum (ABIN350787) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

Publications

Publications: Kinsella, Erdman, Maltese: "Carboxyl-terminal isoprenylation of ras-related GTP-binding proteins encoded by rac1, rac2, and ralA." in: The Journal of biological chemistry, Vol. 266, Issue 15, pp. 9786-94, 1991 (PubMed).

Polakis, Weber, Nevins et al.: "Identification of the ral and rac1 gene products, low molecular mass GTP-binding proteins from human platelets." in: The Journal of biological chemistry, Vol. 264, Issue 28, pp. 16383-9, 1989 (PubMed).

Chardin, Tavitian: "Coding sequences of human ralA and ralB cDNAs." in: Nucleic acids research, Vol. 17, Issue 11, pp. 4380, 1989 (PubMed).

Cantor, Urano, Feig: "Identification and characterization of Ral-binding protein 1, a potential downstream target of Ral GTPases." in: Molecular and cellular biology, Vol. 15, Issue 8, pp. 4578-84, 1995 (PubMed).

Wildey, Viggeswarapu, Rim et al.: "Isolation of cDNA clones and tissue expression of rat ral A and ral B GTP-binding proteins." in: Biochemical and biophysical research communications, Vol. 194, Issue 1, pp. 552-9, 1993 (PubMed).

Park, Weinberg: "A putative effector of Ral has homology to Rho/Rac GTPase activating proteins." in: Oncogene, Vol. 11, Issue 11, pp. 2349-55, 1996 (PubMed).

Zhao, Rivkees: "Tissue-specific expression of GTPas RalA and RalB during embryogenesis and regulation by epithelial-mesenchymal interaction." in: Mechanisms of development, Vol. 97, Issue 1-2, pp. 201-4, 2000 (PubMed).

Hillier, Fulton, Fulton et al.: "The DNA sequence of human chromosome 7." in: Nature, Vol. 424, Issue 6945, pp. 157-64, 2003 (PubMed).

Holbourn, Sutton, Evans et al.: "Molecular recognition of an ADP-ribosylating Clostridium botulinum C3 exoenzyme by RalA GTPase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 15, pp. 5357-62, 2005 (PubMed).