Ras-related Protein Ral-A (rala) (AA 150-200) antibody

Details for Product No. ABIN350790
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Antigen
Synonyms 3010001O15Rik, AW322615, Ral, Rasl1, RAL
Epitope
AA 150-200
(7), (6), (5), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus), Mouse (Murine)
(45), (19), (18), (12), (12), (12), (12)
Host
Rabbit
(36), (7), (3), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC)
(34), (14), (10), (3), (2), (1), (1), (1)
Pubmed 9 references available
Quantity 500 µg
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Catalog No. ABIN350790
454.67 $
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Immunogen A synthetic peptide from aa region 150-200 of human RalA conjugated to blue carrier protein was used as the antigen. The antigen is homologous in rat and mouse.
Isotype IgG
Specificity Specific for Ral-A.
Alternative Name RalA
Background The product of this gene belongs to the small GTPase superfamily, Ras family of proteins. GTP-binding proteins mediate thetransmembrane signaling initiated by the occupancy of certain cell surface receptors. This gene encodes a low molecular mass ras-like GTP-binding protein that shares about 50% similarity with other ras proteins.
Subunit: Interacts with RALBP1 via its effector domain. Interacts with EXOC8 and EXOC2. EXOC2 and EXOC8 have overlapping binding sites and compete for RALA binding. Interacts with Clostridium exoenzyme C3. Interacts with RALGPS1.
Subcellular location: Cell membrane, Lipid-anchor, Cytoplasmic side. Also known as: Rala, Ral, Ral-a, MGC48949.
Research Area Cancer, Transcription Factors
Application Notes A concentration of 10-50 µg/ml is recommended.
The optimal concentration should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
General Kinsella, Erdman, Maltese: "Carboxyl-terminal isoprenylation of ras-related GTP-binding proteins encoded by rac1, rac2, and ralA." in: The Journal of biological chemistry, Vol. 266, Issue 15, pp. 9786-94, 1991 (PubMed).

Polakis, Weber, Nevins et al.: "Identification of the ral and rac1 gene products, low molecular mass GTP-binding proteins from human platelets." in: The Journal of biological chemistry, Vol. 264, Issue 28, pp. 16383-9, 1989 (PubMed).

Chardin, Tavitian: "Coding sequences of human ralA and ralB cDNAs." in: Nucleic acids research, Vol. 17, Issue 11, pp. 4380, 1989 (PubMed).

Cantor, Urano, Feig: "Identification and characterization of Ral-binding protein 1, a potential downstream target of Ral GTPases." in: Molecular and cellular biology, Vol. 15, Issue 8, pp. 4578-84, 1995 (PubMed).

Wildey, Viggeswarapu, Rim et al.: "Isolation of cDNA clones and tissue expression of rat ral A and ral B GTP-binding proteins." in: Biochemical and biophysical research communications, Vol. 194, Issue 1, pp. 552-9, 1993 (PubMed).

Park, Weinberg: "A putative effector of Ral has homology to Rho/Rac GTPase activating proteins." in: Oncogene, Vol. 11, Issue 11, pp. 2349-55, 1996 (PubMed).

Zhao, Rivkees: "Tissue-specific expression of GTPas RalA and RalB during embryogenesis and regulation by epithelial-mesenchymal interaction." in: Mechanisms of development, Vol. 97, Issue 1-2, pp. 201-4, 2000 (PubMed).

Hillier, Fulton, Fulton et al.: "The DNA sequence of human chromosome 7. ..." in: Nature, Vol. 424, Issue 6945, pp. 157-64, 2003 (PubMed).

Holbourn, Sutton, Evans et al.: "Molecular recognition of an ADP-ribosylating Clostridium botulinum C3 exoenzyme by RalA GTPase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 15, pp. 5357-62, 2005 (PubMed).

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