Rho related GTP binding protein RhoF ( region) (Internal Region) antibody

Antigen: Rho related GTP binding protein RhoF ( region)

Binding Site: Internal Region

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350796

Additional Information

UniProt: Q9HBH0

Immunogen: A synthetic peptide from the internal region of human Rho related GTP-binding protein RhoF (Rho family GTPase Rif, ARHF, RIF, Rho in filopodia) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat and mouse.

Format: Lyophilized

Isotype: IgG

Description: Function: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin-based morphology. Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic sidePotential. Cytoplasm › cytoskeleton. Also known as: Rho family GTPase Rif, Rho in filopodia, ARHF, RIF, Rho in filopodia.

Specificity: Appears to be specific for RhOF.

Application Details

Application Notes: IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Purity: IgG

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Ellis, Mellor: "The novel Rho-family GTPase rif regulates coordinated actin-based membrane rearrangements." in: Current biology : CB, Vol. 10, Issue 21, pp. 1387-90, 2000 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).