RHOB antibody

Antigen: RHOB

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350801

Additional Information

Alternative name: Rho-related GTP-binding protein RhoB (ARH6, ARHB, H6)

UniProt: P62745

Immunogen: A synthetic peptide as a part of human, rat, mouse Rho-related GTP-binding protein RhoB (ARH6, ARHB, H6) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. SUBUNIT: Binds ROCK1 and ROCK2. Also binds PKN1/PRK1. Interacts with ARHGEF3 and AKAP13. Interacts with RTKN. SUBCELLULAR LOCATION: Late endosome membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Nucleus. Note=Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus. INDUCTION: By UV irradiation, N-methyl-N-nitrosourea, cisplatin, cyclohexamide and serum stimulation. Also known as: H6, ARH6, ARHB, RHOH6.

Specificity: Appears to be specific for RhOB.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

IF on 1 day- old mouse cortical cell culture using Rabbit antibody to Rho-related GTP-binding protein RhoB (ARH6, ARHB, H6): whole serum (ABIN350801) at 1: 2000 dilution. DAPI counter stained.

Publications

Fritz, Kaina, Aktories: "The ras-related small GTP-binding protein RhoB is immediate-early inducible by DNA damaging treatments." in: The Journal of biological chemistry, Vol. 270, Issue 42, pp. 25172-7, 1995 (PubMed).

Reid, Furuyashiki, Ishizaki et al.: "Rhotekin, a new putative target for Rho bearing homology to a serine/threonine kinase, PKN, and rhophilin in the rho-binding domain." in: The Journal of biological chemistry, Vol. 271, Issue 23, pp. 13556-60, 1996 (PubMed).

Nakamura, Asano, Shindo-Okada et al.: "Cloning of the RhoB gene from the mouse genome and characterization of its promoter region." in: Biochemical and biophysical research communications, Vol. 226, Issue 3, pp. 688-94, 1996 (PubMed).

Liu, Rane, Liu et al.: "RhoB is dispensable for mouse development, but it modifies susceptibility to tumor formation as well as cell adhesion and growth factor signaling in transformed cells." in: Molecular and cellular biology, Vol. 21, Issue 20, pp. 6906-12, 2001 (PubMed).

Liu, Catalfamo, Li et al.: "IL-15 mimics T cell receptor crosslinking in the induction of cellular proliferation, gene expression, and cytotoxicity in CD8+ memory T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 9, pp. 6192-7, 2002 (PubMed).

Malcolm, Ettehadieh, Sadowski: "Mitogen-responsive expression of RhoB is regulated by RNA stability." in: Oncogene, Vol. 22, Issue 40, pp. 6142-50, 2003 (PubMed).

Adini, Rabinovitz, Sun et al.: "RhoB controls Akt trafficking and stage-specific survival of endothelial cells during vascular development." in: Genes & development, Vol. 17, Issue 21, pp. 2721-32, 2003 (PubMed).

Westmark, Bartleson, Malter: "RhoB mRNA is stabilized by HuR after UV light." in: Oncogene, Vol. 24, Issue 3, pp. 502-11, 2005 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).

Ballif, Carey, Sunyaev et al.: "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." in: Journal of proteome research, Vol. 7, Issue 1, pp. 311-8, 2008 (PubMed).