RHOB antibody

Details for Product No. ABIN350801
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Antigen
Synonyms ARH6, ARHB, MST081, MSTP081, RHOH6, Arhb, RHO, AA017882, Arh6
Reactivity
Human, Rat (Rattus), Mouse (Murine)
(51), (30), (29), (12), (12)
Host
Rabbit
(45), (3), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(40), (16), (10), (10), (5), (5), (2), (1), (1)
Pubmed 10 references available
Quantity 100 μL
Options
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Catalog No. ABIN350801
454.67 $
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Immunogen A synthetic peptide from human Rho-related GTP-binding protein RhoB conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in mouse and rat.
Specificity Specific for RhOB.
Purification Whole serum
Background Function: Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development.
Subunit: Binds ROCK1 and ROCK2. Also binds PKN1/PRK1. Interacts with ARHGEF3 and AKAP13. Interacts with RTKN.
Subcellular location: Late endosome membrane, Lipid-anchor. Cell membrane, Lipid-anchor. Nucleus. Note=Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus. INDUCTION: By UV irradiation, N-methyl-N-nitrosourea, cisplatin, cyclohexamide and serum stimulation. Also known as: H6, ARH6, ARHB, RHOH6.
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Supplier Images
anti-RHOB antibody IF on 1 day- old mouse cortical cell culture using Rabbit antibody to Rho-related GTP-binding protein RhoB (ARH6, ARHB, H6): whole serum (ABIN350801) at 1: 2000 dilution. DAPI counter stained.
General Fritz, Kaina, Aktories: "The ras-related small GTP-binding protein RhoB is immediate-early inducible by DNA damaging treatments." in: The Journal of biological chemistry, Vol. 270, Issue 42, pp. 25172-7, 1995 (PubMed).

Reid, Furuyashiki, Ishizaki et al.: "Rhotekin, a new putative target for Rho bearing homology to a serine/threonine kinase, PKN, and rhophilin in the rho-binding domain." in: The Journal of biological chemistry, Vol. 271, Issue 23, pp. 13556-60, 1996 (PubMed).

Nakamura, Asano, Shindo-Okada et al.: "Cloning of the RhoB gene from the mouse genome and characterization of its promoter region." in: Biochemical and biophysical research communications, Vol. 226, Issue 3, pp. 688-94, 1996 (PubMed).

Liu, Rane, Liu et al.: "RhoB is dispensable for mouse development, but it modifies susceptibility to tumor formation as well as cell adhesion and growth factor signaling in transformed cells." in: Molecular and cellular biology, Vol. 21, Issue 20, pp. 6906-12, 2001 (PubMed).

Liu, Catalfamo, Li et al.: "IL-15 mimics T cell receptor crosslinking in the induction of cellular proliferation, gene expression, and cytotoxicity in CD8+ memory T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 9, pp. 6192-7, 2002 (PubMed).

Malcolm, Ettehadieh, Sadowski: "Mitogen-responsive expression of RhoB is regulated by RNA stability." in: Oncogene, Vol. 22, Issue 40, pp. 6142-50, 2003 (PubMed).

Adini, Rabinovitz, Sun et al.: "RhoB controls Akt trafficking and stage-specific survival of endothelial cells during vascular development." in: Genes & development, Vol. 17, Issue 21, pp. 2721-32, 2003 (PubMed).

Westmark, Bartleson, Malter: "RhoB mRNA is stabilized by HuR after UV light." in: Oncogene, Vol. 24, Issue 3, pp. 502-11, 2005 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome. ..." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).

Ballif, Carey, Sunyaev et al.: "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." in: Journal of proteome research, Vol. 7, Issue 1, pp. 311-8, 2008 (PubMed).

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