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Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
|10 references available|
|Quantity||100 µl (Variants)|
|Price||454.67 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 7 to 8 Business Days|
|Alternative name||Rho-related GTP-binding protein RhoB|
|Immunogen||A synthetic peptide as a part of human, rat, mouse Rho-related GTP-binding protein RhoB (ARH6, ARHB, H6) conjugated to an immunogenic carrier protein was used as the immunogen.|
|Description||FUNCTION: Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. SUBUNIT: Binds ROCK1 and ROCK2. Also binds PKN1/PRK1. Interacts with ARHGEF3 and AKAP13. Interacts with RTKN. SUBCELLULAR LOCATION: Late endosome membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Nucleus. Note: Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus. INDUCTION: By UV irradiation, N-methyl-N-nitrosourea, cisplatin, cyclohexamide and serum stimulation. Also known as: H6, ARH6, ARHB, RHOH6, MST081, MSTP081.|
|Specificity||Appears to be specific for RhOB.|
|Application Notes||IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user.|
|Storage||Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.|
|Restrictions||For Research Use only|
Fritz, Kaina, Aktories: "The ras-related small GTP-binding protein RhoB is immediate-early inducible by DNA damaging treatments." in: The Journal of biological chemistry, Vol. 270, Issue 42, pp. 25172-7, 1995 (PubMed).
Reid, Furuyashiki, Ishizaki et al.: "Rhotekin, a new putative target for Rho bearing homology to a serine/threonine kinase, PKN, and rhophilin in the rho-binding domain." in: The Journal of biological chemistry, Vol. 271, Issue 23, pp. 13556-60, 1996 (PubMed).
Nakamura, Asano, Shindo-Okada et al.: "Cloning of the RhoB gene from the mouse genome and characterization of its promoter region." in: Biochemical and biophysical research communications, Vol. 226, Issue 3, pp. 688-94, 1996 (PubMed).
Liu, Rane, Liu et al.: "RhoB is dispensable for mouse development, but it modifies susceptibility to tumor formation as well as cell adhesion and growth factor signaling in transformed cells." in: Molecular and cellular biology, Vol. 21, Issue 20, pp. 6906-12, 2001 (PubMed).
Liu, Catalfamo, Li et al.: "IL-15 mimics T cell receptor crosslinking in the induction of cellular proliferation, gene expression, and cytotoxicity in CD8+ memory T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 9, pp. 6192-7, 2002 (PubMed).
Malcolm, Ettehadieh, Sadowski: "Mitogen-responsive expression of RhoB is regulated by RNA stability." in: Oncogene, Vol. 22, Issue 40, pp. 6142-50, 2003 (PubMed).
Adini, Rabinovitz, Sun et al.: "RhoB controls Akt trafficking and stage-specific survival of endothelial cells during vascular development." in: Genes & development, Vol. 17, Issue 21, pp. 2721-32, 2003 (PubMed).
Westmark, Bartleson, Malter: "RhoB mRNA is stabilized by HuR after UV light." in: Oncogene, Vol. 24, Issue 3, pp. 502-11, 2005 (PubMed).
Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).
Ballif, Carey, Sunyaev et al.: "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." in: Journal of proteome research, Vol. 7, Issue 1, pp. 311-8, 2008 (PubMed).
|Hosts||Rabbit (21), Mouse (3), Sheep (2)|
|Reactivities||Human (21), Mouse (Murine) (9), Rat (Rattus) (9)|
|Applications||Western Blotting (WB) (23), ELISA (14), Immunohistochemistry (IHC) (9), Immunofluorescence (IF) (4), Immunohistochemistry (Fixed) (IHC (fx)) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Dot Blot (Dot) (1)|
|Epitopes||Internal Region (7), Center (2), Internal Region,AA 103-137 (1), Middle Region (1)|