RhoU (CDC42 like GTPase 1, CDC42L1) (Internal Region) antibody

Antigen: RhoU (CDC42 like GTPase 1, CDC42L1)

Binding Site: Internal Region

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350806

Additional Information

UniProt: Q7L0Q8

Immunogen: A synthetic peptide from the internal region of human Rho related GTP binding protein RhoU (CDC42 like GTPase 1, CDC42L1, Ryu GTPase) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen shares 90% identity with rat and mouse sequence.

Format: Lyophilized

Isotype: IgG

Description: Function: Acts upstream of PAK1 to regulate the actin cytoskeleton, adhesion turnover and increase cell migration. Stimulates quiescent cells to reenter the cell cycle. Has no detectable GTPase activity but its high intrinsic guanine nucleotide exchange activity suggests it is constitutively GTP-bound. Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor. Cell junction › focal adhesion. Cell projection › podosome. Note: Localizes to podosomes in SRC-transformed cells. Tissue specificity: Ubiquitously expressed in all tissues examined. Expressed at high levels in the stomach, small intestine, brain, skeletal muscle and placenta. Also known as: Rho related GTP binding protein RhoU, RhoU, Ryu GTPase, CDC42-like GTPase 1, Rho GTPase-like protein ARHU, GTP-binding protein-like 1, ARHU, CDC42L1, G28K, WRCH1, Wnt-1 responsive Cdc42 homolog 1.

Specificity: Appears to be specific for RhoU.

Application Details

Application Notes: IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Purity: IgG

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Tao, Pennica, Xu et al.: "Wrch-1, a novel member of the Rho gene family that is regulated by Wnt-1." in: Genes & development, Vol. 15, Issue 14, pp. 1796-807, 2001 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Gregory, Barlow, McLay et al.: "The DNA sequence and biological annotation of human chromosome 1." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006 (PubMed).

Bubb, Bovee, Buckley et al.: "Scan of human genome reveals no new Loci under ancient balancing selection." in: Genetics, Vol. 173, Issue 4, pp. 2165-77, 2006 (PubMed).

Ory, Brazier, Blangy: "Identification of a bipartite focal adhesion localization signal in RhoU/Wrch-1, a Rho family GTPase that regulates cell adhesion and migration." in: Biology of the cell / under the auspices of the European Cell Biology Organization, Vol. 99, Issue 12, pp. 701-16, 2007 (PubMed).