Ras Homolog Gene Family, Member V (RHOV) (C-Term) antibody

Antigen: Ras Homolog Gene Family, Member V (RHOV)

Synonyms: CHP, ARHV, WRCH2, Chp, Arhv, MGC93183, MGC29297, A030005A06Rik, RHOV, sb:cb832, MGC111951, MGC174618, zgc:111951, si:ch211-244p18.1, chp, arhv, wrch2

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350807

Additional Information

Alternative name: RHOV (CDC42-like GTPase 2) (c-terminal region)

Swiss-Prot: Q96L33

Immunogen: A synthetic peptide from the c-terminal region of human RHOV (CDC42-like GTPase 2) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat and mouse.

Format: Lyophilized

Description: Function: Plays a role in the control of the actin cytoskeleton via activation of the JNK pathway. Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic side. Endosome membrane; Lipid-anchor; Cytoplasmic side. Note: Treatment with TNF activates endosomal but not plasma membrane RHOV Tissue specificity: Highly expressed in pancreas, placenta, and fetal brain. Also known as: Rho-related GTP-binding protein RhoV, Wnt-1 responsive Cdc42 homolog 2, WRCH-2, CDC42-like GTPase 2, GTP-binding protein-like 2, Rho GTPase-like protein ARHV.

Specificity: Appears to be specific for RhoU.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Aronheim, Broder, Cohen et al.: "Chp, a homologue of the GTPase Cdc42Hs, activates the JNK pathway and is implicated in reorganizing the actin cytoskeleton." in: Current biology : CB, Vol. 8, Issue 20, pp. 1125-8, 1999 (PubMed).

Wherlock, Mellor: "The Rho GTPase family: a Racs to Wrchs story." in: Journal of cell science, Vol. 115, Issue Pt 2, pp. 239-40, 2002 (PubMed).

Katoh: "Molecular cloning and characterization of WRCH2 on human chromosome 15q15." in: International journal of oncology, Vol. 20, Issue 5, pp. 977-82, 2002 (PubMed).

Chenette, Abo, Der: "Critical and distinct roles of amino- and carboxyl-terminal sequences in regulation of the biological activity of the Chp atypical Rho GTPase." in: The Journal of biological chemistry, Vol. 280, Issue 14, pp. 13784-92, 2005 (PubMed).

Chenette, Mitin, Der: "Multiple sequence elements facilitate Chp Rho GTPase subcellular location, membrane association, and transforming activity." in: Molecular biology of the cell, Vol. 17, Issue 7, pp. 3108-21, 2006 (PubMed).