Runt-Related Transcription Factor 3 (RUNX3) (internal) antibody

Antigen: Runt-Related Transcription Factor 3 (RUNX3)

Synonyms: AML2, CBFA3, PEBP2aC, FLJ34510, MGC16070, Cbfa3, Pebp2a3, runxb, RUNX3, frrunx3, runx3, aml2, cbfa3, pebp2ac

Epitope: internal

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350810

Additional Information

Alternative name: Runt related transcription factor 3 (Runx3) (internal region)

Swiss-Prot: Q91ZK1

Immunogen: A synthetic peptide from the internal part of rat Runt-related transcription factor 3 (Runx3) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in mouse.

Format: Lyophilized

Description: FUNCTION: Binds to the core site of murine Leukemia virus, the core sequences in the enhancer of the polyomavirus, and also to the enhancers of the T-cell receptor genes. May be involved in the control of cellular proliferation and/or differentiation. SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1. SUBCELLULAR LOCATION: Nucleus. Also known as: Runx3, Core-binding factor, alpha 3 subunit, CBF-alpha 3, Acute myeloid leukemia 2 protein, Oncogene AML-2, Polyomavirus enhancer-binding protein 2 alpha C subunit, PEBP2-alpha C, PEA2-alpha C, SL3-3 enhancer factor 1 alpha C subunit, SL3/AKV core-binding factor alpha C subunit.

Specificity: Appears to be specific for Runx3.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

IF on 1 day- old mouse cortical cell culture using Rabbit antibody to internal part of Runt-related transcription factor 3 (Runx3): whole serum (ABIN350810) at 1: 2000 dilution.

IF on 1 day- old mouse cortical cell culture using internal part of Runt-related transcription factor 3 (Runx3) (ABIN350810) at 1: 2000 dilution.

IF on mouse cerebellum (4% PFA fixed, frozen section) at 1 : 500 dilution using internal part of Runt-related transcription factor 3 (Runx3) (ABIN350810). DAPI counterstained appearing in blue.

Publications

Publications: Calabi, Rhodes, Williamson et al.: "Identification and chromosomal mapping of a third mouse runt-like locus." in: Genomics, Vol. 26, Issue 3, pp. 607-10, 1995 (PubMed).

Wijmenga, Speck, Dracopoli et al.: "Identification of a new murine runt domain-containing gene, Cbfa3, and localization of the human homolog, CBFA3, to chromosome 1p35-pter." in: Genomics, Vol. 26, Issue 3, pp. 611-4, 1995 (PubMed).

Bangsow, Rubins, Glusman et al.: "The RUNX3 gene--sequence, structure and regulated expression." in: Gene, Vol. 279, Issue 2, pp. 221-32, 2001 (PubMed).

Yarmus, Woolf, Bernstein et al.: "Groucho/transducin-like Enhancer-of-split (TLE)-dependent and -independent transcriptional regulation by Runx3." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 19, pp. 7384-9, 2006 (PubMed).