Runt-Related Transcription Factor 1 (RUNX1) antibody

Details for Product No. ABIN350812
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Antigen
Synonyms RUNX1, runx1, AI462102, AML1, Cbfa2, Pebp2a2, Pebpa2b, AML1-EVI-1, AMLCR1, CBFA2, EVI-1, PEBP2aB, runxa, Runx-1, XAML, Xaml1, aml, aml-1, aml1, aml1-evi-1, amlcr1, cbfa2, evi-1, pebp2ab, Aml1
Reactivity
Human, Rat (Rattus), Mouse (Murine)
(213), (106), (99), (42), (42), (24), (12), (5), (4), (3), (2), (2)
Host
Sheep
(177), (41), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(169), (108), (47), (40), (36), (35), (20), (11), (2), (2), (1)
Pubmed 10 references available
Quantity 500 µg
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Catalog No. ABIN350812
454.67 $
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Immunogen A synthetic peptide from human Runt-related transcription factor 1 (Runx1, AML1, CBFA2) conjugated to an immunogenic carrier protein was used as the antigen.
Isotype IgG
Specificity Specific for Runx1.
Alternative Name Runx1
Background Function: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.
Subunit: Heterodimer with CBFB. RUNX1 binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Isoform AML-1L can neither bind DNA nor heterodimerize. Interacts with TLE1 and THOC4. Interacts with ELF1, ELF2 and SPI1. Interacts via its Runt domain with the ELF4 N-terminal region. Interaction with ELF2 isoform 2 (NERF-1a) may act to repress RUNX1-mediated transactivation. Interacts with MYST3 and MYST4. Interacts with SUV39H1, leading to abrogate the transactivating and DNA-binding properties of RUNX1.
Tissue specificity: Highest levels in thymus, bone marrow and peripheral blood. Also known as: Runx1, Core-binding factor, alpha 2 subunit, CBF-alpha 2, Acute myeloid leukemia 1 protein, Oncogene AML-1, Polyomavirus enhancer-binding protein 2 alpha B subunit, PEBP2-alpha B, PEA2-alpha B, SL3-3 enhancer factor 1 alpha B subunit, SL3/AKV core-binding factor alpha B subunit, AML1, CBFA2.
Application Notes A concentration of 10-50 µg/ml is recommended.
The optimal concentration should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
General Daga, Tighe, Calabi: "Leukaemia/Drosophila homology." in: Nature, Vol. 356, Issue 6369, pp. 484, 1992 (PubMed).

Miyoshi, Shimizu, Kozu et al.: "t(8;21) breakpoints on chromosome 21 in acute myeloid leukemia are clustered within a limited region of a single gene, AML1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 23, pp. 10431-4, 1992 (PubMed).

Levanon, Negreanu, Bernstein et al.: "AML1, AML2, and AML3, the human members of the runt domain gene-family: cDNA structure, expression, and chromosomal localization." in: Genomics, Vol. 23, Issue 2, pp. 425-32, 1995 (PubMed).

Zhu, Yeadon, Burden: "AML1 is expressed in skeletal muscle and is regulated by innervation." in: Molecular and cellular biology, Vol. 14, Issue 12, pp. 8051-7, 1994 (PubMed).

Bae, Yamaguchi-Iwai, Ogawa et al.: "Isolation of PEBP2 alpha B cDNA representing the mouse homolog of human acute myeloid leukemia gene, AML1." in: Oncogene, Vol. 8, Issue 3, pp. 809-14, 1993 (PubMed).

Nucifora, Birn, Espinosa et al.: "Involvement of the AML1 gene in the t(3;21) in therapy-related leukemia and in chronic myeloid leukemia in blast crisis." in: Blood, Vol. 81, Issue 10, pp. 2728-34, 1993 (PubMed).

Ghozi, Bernstein, Negreanu et al.: "Expression of the human acute myeloid leukemia gene AML1 is regulated by two promoter regions." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 5, pp. 1935-40, 1996 (PubMed).

Zhang, Bae, Huang et al.: "A novel transcript encoding an N-terminally truncated AML1/PEBP2 alphaB protein interferes with transactivation and blocks granulocytic differentiation of 32Dcl3 myeloid cells." in: Molecular and cellular biology, Vol. 17, Issue 7, pp. 4133-45, 1997 (PubMed).

Tahirov, Inoue-Bungo, Morii et al.: "Structural analyses of DNA recognition by the AML1/Runx-1 Runt domain and its allosteric control by CBFbeta." in: Cell, Vol. 104, Issue 5, pp. 755-67, 2001 (PubMed).

Baeckstroem, Wolf-Watz, Grundstroem et al.: "The RUNX1 Runt domain at 1.25A resolution: a structural switch and specifically bound chloride ions modulate DNA binding." in: Journal of molecular biology, Vol. 322, Issue 2, pp. 259-72, 2002 (PubMed).

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