Secretory Carrier Membrane Protein 1 (SCAMP1) (1t cytoplasmic domain) antibody

Antigen: Secretory Carrier Membrane Protein 1 (SCAMP1)

Synonyms: SCAMP, SCAMP37, AI415563, AW122395, 4930505M11Rik, MGC77214, zgc:77214, SCAMP1, MGC127646, scamp1, DKFZp459H1724

Epitope: 1t cytoplasmic domain

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350820

Additional Information

Alternative name: SCAMP1 (Secretory carrier associated membrane protein 1) (1t cytoplasmic domain)

Swiss-Prot: O15126

Immunogen: A synthetic peptide from the 1t cytoplasmic domain of human SCAMP1 (Secretory carrier associated membrane protein 1) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat and mouse.

Format: Lyophilized

Description: FUNCTION: Functions in post-Golgi recycling pathways. Acts as a recycling carrier to the cell surface. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Widely expressed, with highest expression in brain. Also known as: SCAMP-1, SCAMP 1, Secretory carrier-associated membrane protein 1, Secretory carrier membrane protein 1, SCAMP 37.

Specificity: Appears to be specific for SCAMP1.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

IF on 1 day- old mouse cortical cell culture using Rabbit antibody to 1t cytoplasmic domain of SCAMP1 (Secretory carrier associated membrane protein 1): whole serumat 1: 2000 dilution. DAPI counter stained.

IF on 1 day- old mouse cortical cell culture using Rabbit antibody to 1t cytoplasmic domain of SCAMP1 (Secretory carrier associated membrane protein 1): whole serumat 1: 2000 dilution. DAPI counter stained appearing in blue.

Publications

Publications: Brand, Castle: "SCAMP 37, a new marker within the general cell surface recycling system." in: The EMBO journal, Vol. 12, Issue 10, pp. 3753-61, 1993 (PubMed).

Singleton, Wu, Castle: "Three mammalian SCAMPs (secretory carrier membrane proteins) are highly related products of distinct genes having similar subcellular distributions." in: Journal of cell science, Vol. 110 ( Pt 17), pp. 2099-107, 1997 (PubMed).

Mao, Fu, Wu et al.: "Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 14, pp. 8175-80, 1998 (PubMed).

Fernuandez-Chacuon, Achiriloaie, Janz et al.: "SCAMP1 function in endocytosis." in: The Journal of biological chemistry, Vol. 275, Issue 17, pp. 12752-6, 2000 (PubMed).