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SQSTM1 antibody (Sequestosome 1) (AA 300-360)

Details for Product anti-SQSTM1 Antibody No. ABIN350826, Supplier: Log in to see
Antigen
  • A170
  • MGC79491
  • OSF-6
  • Osi
  • OSIL
  • p60
  • p62
  • p62B
  • PDB3
  • sb:cb621
  • sqstm1
  • SQSTM1
  • STAP
  • zgc:85784
  • ZIP
  • ZIP3
Alternatives
anti-Human SQSTM1 antibody for Immunohistochemistry (Frozen Sections)
Epitope
AA 300-360
45
41
19
19
17
17
17
14
14
13
7
6
5
5
5
5
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
241
64
63
3
2
2
1
1
1
Host
Sheep
186
49
4
2
Clonality
Polyclonal
Conjugate
This SQSTM1 antibody is un-conjugated
13
12
12
8
8
8
5
5
5
3
3
2
2
2
2
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
173
91
77
56
49
41
36
36
12
11
9
4
3
2
Supplier
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Immunogen A synthetic peptide from aa region 300-360 of human SQSTM1 has been used as the antigen.
Specificity Specific for sequestosome-1.
Purification Whole serum
Alternative Name SQSTM1 (SQSTM1 Antibody Abstract)
Background Function: Adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels.
Subcellular location: Cytoplasm. Late endosome. Nucleus. Note=Sarcomere. In cardiac muscles localizes to the sarcomeric band. Localizes to late endosomes. May also localize to the nucleus. Accumulates in neurofibrillary tangles and in Lewy bodies of neurons from individuals with Alzheimer and Parkinson disease respectively. Enriched in Rosenthal fibers of pilocytic astrocytoma. In liver cells, accumulates in Mallory bodies associated with alcoholic hepatitis, Wilson disease, indian childhood cirrhosis and in hyaline bodies associated with hepatocellular carcinoma.
Tissue specificity: Ubiquitously expressed. Also known as: Sequestosome 1, phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, ubiquitin-binding protein p62, EBI3-associated protein of 60 kDa, p60, EBIAP, SQSTM1, A170, OSIL, PDB3, ZIP3, p62B.
Pathways NF-kappaB Signaling, Neurotrophin Signaling Pathway
Application Notes Use at a dilution of 1 : 200 to 1 : 2000.
The optimal dilution should be determined by the end user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Olsen, Blagoev, Gnad, Macek, Kumar, Mortensen, Mann: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Nousiainen, Silljé, Sauer, Nigg, Körner: "Phosphoproteome analysis of the human mitotic spindle." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 14, pp. 5391-6, 2006 (PubMed).

Good, Busfield, Fletcher, Lovelock, Duffy, Kesting, Andersen, Shaw: "Identification of SQSTM1 mutations in familial Paget's disease in Australian pedigrees." in: Bone, Vol. 35, Issue 1, pp. 277-82, 2004 (PubMed).

Hocking, Lucas, Daroszewska, Cundy, Nicholson, Donath, Walsh, Finlayson, Cavey, Ciani, Sheppard, Searle, Layfield, Ralston: "Novel UBA domain mutations of SQSTM1 in Paget's disease of bone: genotype phenotype correlation, functional analysis, and structural consequences." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 19, Issue 7, pp. 1122-7, 2004 (PubMed).

Ciani, Layfield, Cavey, Sheppard, Searle: "Structure of the ubiquitin-associated domain of p62 (SQSTM1) and implications for mutations that cause Paget's disease of bone." in: The Journal of biological chemistry, Vol. 278, Issue 39, pp. 37409-12, 2003 (PubMed).

Hocking, Lucas, Daroszewska, Mangion, Olavesen, Cundy, Nicholson, Ward, Bennett, Wuyts, Van Hul, Ralston: "Domain-specific mutations in sequestosome 1 (SQSTM1) cause familial and sporadic Paget's disease." in: Human molecular genetics, Vol. 11, Issue 22, pp. 2735-9, 2002 (PubMed).

Stumptner, Heid, Fuchsbichler, Hauser, Mischinger, Zatloukal, Denk: "Analysis of intracytoplasmic hyaline bodies in a hepatocellular carcinoma. Demonstration of p62 as major constituent." in: The American journal of pathology, Vol. 154, Issue 6, pp. 1701-10, 1999 (PubMed).

Devergne, Hummel, Koeppen, Le Beau, Nathanson, Kieff, Birkenbach: "A novel interleukin-12 p40-related protein induced by latent Epstein-Barr virus infection in B lymphocytes." in: Journal of virology, Vol. 70, Issue 2, pp. 1143-53, 1996 (PubMed).

Joung, Strominger, Shin: "Molecular cloning of a phosphotyrosine-independent ligand of the p56lck SH2 domain." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 12, pp. 5991-5, 1996 (PubMed).

Vadlamudi, Joung, Strominger, Shin: "p62, a phosphotyrosine-independent ligand of the SH2 domain of p56lck, belongs to a new class of ubiquitin-binding proteins." in: The Journal of biological chemistry, Vol. 271, Issue 34, pp. 20235-7, 1996 (PubMed).