Sequestosome 1 (SQSTM1) antibody

Antigen: Sequestosome 1 (SQSTM1)

Synonyms: p60, p62, A170, OSIL, PDB3, ZIP3, p62B, Osi, STAP, OSF-6, SQSTM1, MGC127197, MGC64432, sb:cb621, zgc:85784, DKFZp468L2213, sqstm1, MGC79491

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN350828

Additional Information

Alternative name: sequestosome-1 (SQSTM1, ubiquitin-binding protein p62, p60, EBIAP, PDB3, ZIP3)

UniProt: Q13501

Immunogen: A synthetic peptide as a part of sequestosome-1 (ubiquitin-binding protein p62, p60, EBIAP) conjugated to immunogenic carrier protein has been used as the immunogen.

Format: Lyophilized

Isotype: IgG

Description: FUNCTION: Adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. SUBCELLULAR LOCATION: Cytoplasm. Late endosome. Nucleus. Note=Sarcomere. In cardiac muscles localizes to the sarcomeric band. Localizes to late endosomes. May also localize to the nucleus. Accumulates in neurofibrillary tangles and in Lewy bodies of neurons from individuals with Alzheimer and Parkinson disease respectively. Enriched in Rosenthal fibers of pilocytic astrocytoma. In liver cells, accumulates in Mallory bodies associated with alcoholic hepatitis, Wilson disease, indian childhood cirrhosis and in hyaline bodies associated with hepatocellular carcinoma. TISSUE SPECIFICITY: Ubiquitously expressed. Also known as: Sequestosome 1, phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, ubiquitin-binding protein p62, EBI3-associated protein of 60 kDa, p60, EBIAP, SQSTM1, A170, OSIL, PDB3, ZIP3, p62B.

Specificity: Appears to be specific for sequestosome-1.

Application Details

Application Notes: IHC (both on frozen and paraffin embedded tissues), WB. Use at a concentration of 10-50 µg/ml. The optimal concentration should be determined by the end user.

Purity: IgG

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Devergne, Hummel, Koeppen et al.: "A novel interleukin-12 p40-related protein induced by latent Epstein-Barr virus infection in B lymphocytes." in: Journal of virology, Vol. 70, Issue 2, pp. 1143-53, 1996 (PubMed).

Joung, Strominger, Shin: "Molecular cloning of a phosphotyrosine-independent ligand of the p56lck SH2 domain." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 12, pp. 5991-5, 1996 (PubMed).

Vadlamudi, Joung, Strominger et al.: "p62, a phosphotyrosine-independent ligand of the SH2 domain of p56lck, belongs to a new class of ubiquitin-binding proteins." in: The Journal of biological chemistry, Vol. 271, Issue 34, pp. 20235-7, 1996 (PubMed).

Stumptner, Heid, Fuchsbichler et al.: "Analysis of intracytoplasmic hyaline bodies in a hepatocellular carcinoma. Demonstration of p62 as major constituent." in: The American journal of pathology, Vol. 154, Issue 6, pp. 1701-10, 1999 (PubMed).

Hocking, Lucas, Daroszewska et al.: "Domain-specific mutations in sequestosome 1 (SQSTM1) cause familial and sporadic Paget's disease." in: Human molecular genetics, Vol. 11, Issue 22, pp. 2735-9, 2002 (PubMed).

Ciani, Layfield, Cavey et al.: "Structure of the ubiquitin-associated domain of p62 (SQSTM1) and implications for mutations that cause Paget's disease of bone." in: The Journal of biological chemistry, Vol. 278, Issue 39, pp. 37409-12, 2003 (PubMed).

Hocking, Lucas, Daroszewska et al.: "Novel UBA domain mutations of SQSTM1 in Paget's disease of bone: genotype phenotype correlation, functional analysis, and structural consequences." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 19, Issue 7, pp. 1122-7, 2004 (PubMed).

Good, Busfield, Fletcher et al.: "Identification of SQSTM1 mutations in familial Paget's disease in Australian pedigrees." in: Bone, Vol. 35, Issue 1, pp. 277-82, 2004 (PubMed).

Nousiainen, Silljé, Sauer et al.: "Phosphoproteome analysis of the human mitotic spindle." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 14, pp. 5391-6, 2006 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).